Keeping up with the news that surrounds CRISPR technology can be overwhelming. We’ve got you covered – we’ve picked out 5 of the biggest and most exciting CRISPR stories of the past week to keep you up to date without too much effort. This week we tell you about the new CRISPR mouse model that could speed up colon cancer research, the live animals that CRISPR has eliminated HIV from, and how Amazon’s Alexa might make lab life easier in the future.
CRISPR/Cas9 technology relies on two critical components; the Cas9 endonuclease protein that cuts DNA, and the guide RNA (gRNA) that directs Cas9 to its target sequence. The gRNA includes a sequence of 17-20 nucleotides that is unique and tailored to the locus of interest.
The task of designing, synthesizing and delivering these distinct gRNAs can be done by researchers in their own labs; a process that has been implemented for years.
Until the recent emergence of synthetic single guide RNAs (sgRNA), the in vitro transcription (IVT) approach was the most common method. IVT relies on a kit to produce a DNA template to make gRNA, which is then integrated into cells. When IVT was first introduced, it was quick and cheap when compared to previously used methods such as plasmid-based approaches. However, new technology has enabled the production of high efficiency, cost-effective synthetic gRNAs which have surpassed the, now outdated, IVT methods.
The best podcasts covering CRISPR that we could find. From beginners to seasoned researchers, there’s a podcast for everyone. With a multitude of guests and viewpoints, this list contains everything you need to know about CRISPR technology.
The rapid pace of innovation in the CRISPR field can often make it difficult to discern which new developments are in fact improvements to the state of the art. An excellent criterion for judging improvements in the field asks the question: does a tool holistically improve quality and ease-of-use across the board, without any significant drawbacks?
With this question in mind, we explore the possibility of adding electroporation enhancer carrier DNA to improve transfection efficiencies in genome editing systems.
Keeping up with the news that surrounds CRISPR technology can be overwhelming. We’ve got you covered – we’ve picked out 5 of the biggest and most exciting CRISPR stories of the past week to keep you up to date without too much effort. This week we’ll look at emerging CRISPR applications in various fields such as biomanufacturing and wine making, as well as uncover a so-called “molecular Lego” system that could improve the current CRISPR system.
In the short time since its discovery, CRISPR has enabled tremendous scientific advances that have caught headlines and spotlight around the world. Today we’re looking to the future to see where CRISPR might go in 2017. Like every other year, it’ll shock and stun us with innovative and cutting-edge advances quicker than we ever thought possible, so this is just a starting point.
There are multiple design tools available to help simplify the process of CRISPR guide selection and allow you to get on with your experiments. We’ve profiled 4 of the frequently used CRISPR design tools, and given our thoughts on each. Read on to find out what the benefits of each of these tools are, and to find out which may be most suited to your needs.
As the Holiday season begins, the streets fill with busy shoppers and scientists remain loyal to their lab benches around the world in an effort to get their experiments finished up in time for the holidays.
Is it all starting to get a bit much? Feeling super stressed and wishing you could be out soaking up the Holiday spirit too?
We can’t promise that we will get your experiments finished on time, but we can inject some festive fun for scientists.
Struggling to keep up with the latest news from the world of CRISPR? We’ve got you covered – This week we’re cutting through the noise to look at CRISPR’s potential as a TV star, recent progress in sickle cell therapy, and we’ve even found an intro guide to CRISPR in video form for those of you who aren’t sure what we’re talking about, or need to explain CRISPR quickly and clearly to someone else who isn’t sure what we’re talking about.
Genome editing with CRISPR technology is a powerful method for manipulating the genetic material held inside a multitude of different cell types.
So what’s the big deal about synthetic RNA? Why should you be using it instead of plasmid-derived or in vitro transcribed (IVT) guides? And perhaps most importantly, how do you explain your reasoning to the PI in your lab?
Read on to find out the top five things you should be telling your PI (forward them this blog post and they’ll soon begin to come around to your way of thinking!).