Why Synthetic RNA?

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The benefits of synthetic RNA over plasmid-derived or in vitro transcribed (IVT) guides include faster transfection readiness, greater accuracy and consistency, less laboratory time and lower labor costs. Because synthetic RNA is 100% DNA-free, there’s no risk of integrating foreign DNA into cell lines.

There is no need to design, construct, sequence verify and purify plasmid DNA for every guide you want to test. And there’s no need to spend hours working with expensive IVT kits.

Synthetic RNA has the advantage, particularly when forming a ribonucleoprotein (RNP) complex, of improving editing efficiency and consistency while reducing off-target effects.

The Benefits of Synthetic RNA

Speed

Compared to plasmid-based RNA guides, there is no need to design, construct, sequence verify and purify plasmid DNA for every guide you want to test. And there’s no need to spend precious lab hours working with expensive IVT kits.

Quality

Consistently pure RNA synthesis results in consistent CRISPR genome editing. By controlling the quality and exact amount of RNA for your transfection or microinjection, the highest on-target editing efficiency can be achieved.

Accuracy

When forming RNP complexes with Cas9, you will get the cleanest possible edits. After transfection and genome editing have occurred, Cas9 and exogenous RNA will degrade. The probability of off-target effects are greatly reduced.

Price

Synthetic RNA offers an unbeatable price point when compared to the cost (and labor) of preparing guide RNA through plasmid or IVT methods. Synthego can synthesize cr/tracrRNA and sgRNA at smaller scales than anyone else in the industry – meaning you only pay for what you need.

High Throughput

Synthetic RNA offers the kind of scalability not feasible with other traditional guide RNA generation techniques. Synthetic RNA allows you to order libraries of thousands of guide RNAs to test – avoid huge amounts labor and cost involved in creating guides using plasmids or IVT for high throughput applications!

Easy to Use

Once a target sequence has been selected it’s easy to order RNA oligos through our online portal – which makes it as simple to order RNA for a single target as it does for a hundred. Once you click order, just sit back, relax and wait for your lyophilized RNA to show up in a few days.

CRISPRevolution Synthetic RNA vs. Plasmid and IVT

CRISPRevolution Plasmid IVT
Process 1. Choose target sequence
2. Order synthetic RNA from Synthego
1. Choose target sequence
2. Design/order DNA primers
3. PCR insert
4. Ligate into plasmid
5. Transform into cells
6. Screen cells
7. Sequence verify plasmid
8. Purify plasmid DNA
1. Choose target sequence
2. Design/order DNA primers
3. Assemble guide by PCR
4. Perform IVT
5. Purify guide RNA
Time to Transfection Ready for transfection 7-14 days 1-3 days
Transfection Labor Time Minimal Days of lab work Full day of lab work
Off-target Effects Lowest Variable Variable
Efficiency Up to 90% efficiency Variable Variable
Consistency Highest Variable Variable
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