Four guide RNAs were designed per gene to knockout 3 kinases across the human genome. These were constructed as chemically modified synthetic sgRNA and electroporated into Jurkat cells. The DNA from the resulting pool of cells was analyzed by TIDE analysis, demonstrating a high editing efficiency across the board with multiple guides per gene exceeding 50% editing efficiency. This demonstrates the high editing efficiency of the guides included within the Gene Knockout Kit. See more data.