Pre-designed synthetic guide RNAs for common human T-cell engineering targets. Each variant contains an experimentally tested sequence and is supplied lyophilized at 10 nmol. Use for workflow development, performance benchmarking, or TCR-disruption research. Performance may vary by cell type, delivery method, and experimental conditions. For research use only.
- Reduce upfront screening with pre-designed guides for common T-cell engineering targets
- Experimentally tested in K562 cells to provide a strong starting point
- Convenient 10 nmol lyophilized format for RNP workflow development
- Designed for controls, workflow optimization, and performance benchmarking
SpCas9 mRNA encodes the widely used CRISPR nuclease for efficient genome editing in mammalian cells. Delivery of mRNA enables rapid, transient Cas9 expression that reduces persistent nuclease exposure while supporting robust editing when paired with synthetic sgRNAs in research workflows.
- Enables efficient gene knockout and precise sequence modification
- Pairs with synthetic gRNAs for high on-target editing performance
- Supports transient nuclease expression for controlled editing activity
- Suitable for ex vivo cell engineering and in vivo genome editing research
hfCas12Max mRNA encodes a high-fidelity Cas12 nuclease engineered for precise genome editing with reduced off-target activity. Transient mRNA delivery enables controlled nuclease expression while supporting efficient editing when paired with compatible CRISPR guide RNAs.
- Improves editing precision with reduced off-target activity
- Expands genomic access through broad PAM compatibility
- Supports transient, controlled nuclease expression
- Designed for ex vivo cell engineering and in vivo editing applications
E. coli High-Yield Cell-Free Protein Expression Kit
Built for diverse needs and high‑performance workflows, this cell-free protein expression kit leverages the Continuous Exchange Cell-Free (CECF) principle along with an enhanced E.coli lysate to achieve medium-to-preparative-scale protein yields from plasmid DNA. It supports efficient and scalable production of up to 6-mg protein per run.
- High-yield CECF system enables optimized plasmid-based expression scalable from 0.5 mg to 6 mg per 1-mL reaction
- Ideal for structural biology workflows including NMR spectroscopy and X-ray crystallography thanks to a preparative-scale yield
- Flexible amino acid supply allows substitution of methionine for labeling studies
- Optimized for thermomixer-based operation, ensuring consistent CECF exchange and robust yields
E. coli Cell-Free Protein Expression Kit
Go from genes to proteins in hours. This fast cell‑free protein synthesis system uses optimized E. coli lysates and supports both circular and linear DNA templates to produces up to 20 µg of functional protein in ~4 hours. Ideal for rapid screening, parallel expression, and workflow scaling in tubes or microplates.
- High yield E. coli lysate drives efficient coupled transcription/translation, generating up to 20-µg protein per 50-µL reaction in ~4 h
- Supports linear and plasmid DNA for rapid construct screening without cloning, improving early expression workflows
- Flexible format works in microplates or tubes, enabling scalable, parallelized protein production
- Suitable for optimization studies, including chaperone or additive screening to enhance active protein yield
This validated controls kit is engineered for SpCas9 gene editing in human cells. It enables the precise assessment of cleavage efficiency and specificity by providing benchmark positive and negative gRNAs targeting a known locus. These controls are essential for optimizing transfection or electroporation protocols, validating sgRNA delivery, and quantifying on-target indel formation for reliable data normalization.
- Validate SpCas9 editing activity with positive and negative controls for human gene targets
- Establish a benchmark for quantifying on-target cleavage efficiency and indel formation
- Normalize experimental data to distinguish delivery failure from ineffective gRNA design
eSpOT-ON is an engineered high-fidelity Cas9 variant, designed for therapeutic gene editing. Delivered in mRNA format, eSpOT-ON provides superior on-target activity with low off-target effects. This nuclease has been validated in preclinical studies and is ready to support your next breakthrough in cell and gene therapy development.
- Engineered for precise and safe genome editing.
- Significantly reduces off-target editing.
- Lowers the risk of chromosomal translocations.
- Validated in a wide range of human cell lines, including iPSCs.