SpCas9 mRNA encodes the widely used CRISPR nuclease for efficient genome editing in mammalian cells. Delivery of mRNA enables rapid, transient Cas9 expression that reduces persistent nuclease exposure while supporting robust editing when paired with synthetic sgRNAs in research workflows.
hfCas12Max mRNA encodes a high-fidelity Cas12 nuclease engineered for precise genome editing with reduced off-target activity. Transient mRNA delivery enables controlled nuclease expression while supporting efficient editing when paired with compatible CRISPR guide RNAs.
Built for diverse needs and high‑performance workflows, this cell-free protein expression kit leverages the Continuous Exchange Cell-Free (CECF) principle along with an enhanced E.coli lysate to achieve medium-to-preparative-scale protein yields from plasmid DNA. It supports efficient and scalable production of up to 6-mg protein per run.
Go from genes to proteins in hours. This fast cell‑free protein synthesis system uses optimized E. coli lysates and supports both circular and linear DNA templates to produces up to 20 µg of functional protein in ~4 hours. Ideal for rapid screening, parallel expression, and workflow scaling in tubes or microplates.
This validated controls kit is engineered for SpCas9 gene editing in human cells. It enables the precise assessment of cleavage efficiency and specificity by providing benchmark positive and negative gRNAs targeting a known locus. These controls are essential for optimizing transfection or electroporation protocols, validating sgRNA delivery, and quantifying on-target indel formation for reliable data normalization.
eSpOT-ON is an engineered high-fidelity Cas9 variant, designed for therapeutic gene editing. Delivered in mRNA format, eSpOT-ON provides superior on-target activity with low off-target effects. This nuclease has been validated in preclinical studies and is ready to support your next breakthrough in cell and gene therapy development.
T4 RNA Ligase II joins 3'-OH RNA to 5'-phosphorylated oligos within duplex contexts, reducing adapter dimers and artifacts. It produces cleaner small RNA libraries and supports dsRNA nick sealing and RNA circularization at various scales.
In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions. Maximize IVT yield by removing PPi product inhibition. Improve full‑length purity and reduce truncated RNA. Simple spike‑in; no buffer changes to your mix. Cost‑efficient: more mRNA per run saves template and NTPs.