eSpOT-ON Recombinant Protein
eSpOT-ON nuclease is an engineered high-fidelity Cas9 protein designed for therapeutic applications. It offers high on-target editing efficiency while minimizing off-target activity, making it a safer choice for precise cell and gene therapy development. This Cas9 protein is optimized for both accuracy and safety, providing a reliable solution for your gene editing needs.
- Engineered for high on-target efficiency and reduced off-target effects.
- Minimizes off-target edits, enhancing safety in therapeutic applications.
- Produces staggered DNA cuts, which can improve DNA integration.
- Validated for safety and specificity in cell and gene therapies
eSpOT-ON is an engineered high-fidelity Cas9 variant, designed for therapeutic gene editing. Delivered in mRNA format, eSpOT-ON provides superior on-target activity with low off-target effects. This nuclease has been validated in preclinical studies and is ready to support your next breakthrough in cell and gene therapy development.
- Engineered for precise and safe genome editing.
- Significantly reduces off-target editing.
- Lowers the risk of chromosomal translocations.
- Validated in a wide range of human cell lines, including iPSCs.
hfcas12max Recombinant Protein
hfCas12Max is a high-fidelity CRISPR nuclease engineered for therapeutic genome editing. It offers high on-target and low off-target editing across various cell types. Its small size and broad PAM sequence recognition make it an ideal choice for both ex vivo and in vivo CRISPR-based therapeutic development, allowing you to edit more of the genome with confidence.
- Engineered for high-fidelity with high on-target and low off-target editing.
- Broad PAM sequence recognition expands the range of editable genomic sites.
- Small size allows for efficient delivery using AAV and lipid nanoparticles.
- Requires a shorter sgRNA, enhancing stability and editing efficiency.
AccuBase™ Cytosine Base Editor
AccuBase™ is an engineered cytosine base editor designed for therapeutic applications. It enables precise single-base conversions from C to T, minimizing off-target activity and eliminating the risk of double-strand breaks. This makes it a safer and more reliable solution for advancing clinical and commercial applications in cell and gene therapy.
- Engineered for high-efficiency and exceptional fidelity.
- Eliminates double-strand breaks, reducing genomic instability.
- Demonstrates remarkably low off-target activity for safer therapeutics.
- Activates only when bound to the DNA target site, preventing unintended edits.
SpCas9 nuclease is a high-quality, recombinant protein that enables robust and reliable genome editing. This widely used CRISPR nuclease, complexed with a synthetic single guide RNA (sgRNA), can achieve high editing efficiencies. It features two nuclear localization signals (2NLS) to enhance its transport into the nucleus for precise gene editing.
Enables high-precision gene editing with a blunt-end cut.
Features two nuclear localization signals (2NLS) for enhanced nuclear transport.
Complexed with sgRNA to achieve high editing efficiencies.
Used in a wide range of applications, including cell and gene therapy.
Pyrophosphatase, Inorganic
In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions.
- Maximize IVT yield by removing PPi product inhibition
- Improve full‑length purity and reduce truncated RNA
- Simple spike‑in; no buffer changes to your mix
- Cost‑efficient: more mRNA per run saves template and NTPs
T4 RNA Ligase II ligates 3′‑OH RNA to 5′‑phosphorylated oligos in a duplex context, suppressing adapter‑dimers and artifacts. It enables cleaner small RNA library preparation and supports dsRNA nick sealing and RNA circularization across scales.
- Duplex‑guided ligation suppresses adapter‑dimers
- Enables cleaner small RNA library profiles
- Supports dsRNA nick sealing and RNA circularization
- Scales from pilot to high‑throughput workflows
Hot-Start DNA Polymerase, High Fidelity
Proofreading hot‑start polymerase delivering higher fidelity than Taq with robust amplification of mid‑length targets. Produces blunt‑end products for cloning and is well‑suited for NGS library amplification.
- 50x higher fidelity than Taq with proofreading
- Robust amplification of up to 5–10 kb targets
- Produces blunt ends ideal for blunt cloning
- Reliable for NGS library amplification
RNase‑free DNase I rapidly digests plasmid templates after IVT and removes genomic DNA from RNA preps. Protect RNA integrity and prevent false positives in RT‑qPCR and RNA‑seq with a high‑activity enzyme compatible with common buffers.
- Rapid post‑IVT template removal
- Clear gDNA for accurate RT‑qPCR and RNA‑seq
- High activity across common buffers and kits
- RNase‑free: preserves RNA integrity