In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions.
T4 RNA Ligase II ligates 3′‑OH RNA to 5′‑phosphorylated oligos in a duplex context, suppressing adapter‑dimers and artifacts. It enables cleaner small RNA libraries and supports dsRNA nick sealing and RNA circularization across scales.
Proofreading hot‑start polymerase delivering higher fidelity than Taq with robust amplification of mid‑length targets. Produces blunt‑end products for cloning and is well‑suited for NGS library amplification.
RNase‑free DNase I rapidly digests plasmid templates after IVT and removes genomic DNA from RNA preps. Protect RNA integrity and prevent false positives in RT‑qPCR and RNA‑seq with a high‑activity enzyme compatible with common buffers.
Broad‑spectrum serine protease rapidly inactivates RNases/DNases and digests proteins. Stable lyophilizate with PCR‑grade purity supports DNA/RNA extraction and cleanup across varied samples and conditions.
Monoclonal antibody reversibly inhibits Taq at ambient temperature and releases during initial denaturation. Reduces primer‑dimers and mis‑priming for cleaner, more specific amplification setups.
Integrates reverse transcription with endpoint RPA for fast RNA detection at low constant temperatures. Lyophilized 2× mix simplifies setup for gel or lateral‑flow analysis in ~10–20 minutes.
Integrates reverse transcription with real-time RPA for rapid RNA target quantification. 2× lyophilized master mix supports easy workflow integration with standard instruments. Real‑time detection via exo probes at 37–42 °C with minimal hands‑on time.
Lyophilized 2× master mix for endpoint detection delivers highly sensitive amplification in ~10–20 minutes without thermal cycling. Streamlined single-step reconstitution for easy integration and rapid assay development.