Built for diverse needs and high‑performance workflows, this cell-free protein expression kit leverages the Continuous Exchange Cell-Free (CECF) principle along with an enhanced E.coli lysate to achieve medium-to-preparative-scale protein yields from plasmid DNA. It supports efficient and scalable production of up to 6-mg protein per run.

• High-yield CECF system enables optimized plasmid-based expression scalable from 0.5 mg to 6 mg per 1-mL reaction.
• Ideal for structural biology workflows including NMR spectroscopy and X-ray crystallography thanks to a preparative-scale yield.
• Flexible amino acid supply allows substitution of methionine for labeling studies.
• Optimized for thermomixer-based operation, ensuring consistent CECF exchange and robust yields.

Go from genes to proteins in hours. This fast cell‑free protein synthesis system uses optimized E. coli lysates and supports both circular and linear DNA templates to produces up to 20 µg of functional protein in 4 hours. Ideal for rapid screening, parallel expression, and workflow scaling in tubes or microplates.

• High yield E. coli lysate drives efficient coupled transcription/translation, generating up to 20 µg protein per 50 µL reaction in 4 h.
• Supports linear and plasmid DNA for rapid construct screening without cloning, improving early expression workflows.
• Flexible format works in microplates or tubes, enabling scalable, parallelized protein production.
• Suitable for optimization studies, including chaperone or additive screening to enhance active protein yield.