CRISPRevolution is a portfolio of synthetic RNA designed for CRISPR genome editing and research. Designed by you and built by Synthego, our products offer economical access to fully synthetic RNA for high fidelity editing and increased precision in genome editing.
EZ RNA Kits
Designed for simple, consistent CRISPR gene edits with a fast turnaround and low cost. Available in crRNA:tracrRNA or sgRNA formats.
Gene knockout using a CRISPRevolution EZ RNA Kit with Cas9 Gene Editing Nuclease Protein is simple:
A CRISPR design tool (for example, MIT CRISPR Designer) is used to create a target sequence within a region of interest in the genome. This target sequence is then used to order a CRISPRevolution EZ RNA Kit.
Genomic editing with Cas9 gene editing nuclease protein and CRISPRevolution EZ Kit guide RNA is performed by co-transfecting all components with a transfection reagent.
One may then observe phenotypes directly, or undertake a genetic screen to estimate gene editing efficiency prior to clonal cell line generation and characterization.
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