Chemically Modified Synthetic RNA

Essential for CRISPR/Cas9 Editing in Primary and Stem Cells

Chemically modified synthetic sgRNAs provide superior CRISPR/Cas9 editing in primary cells, stem cells, and challenging cell lines (such as K562, cancer cell lines and those of hematopoietic origin) by providing protection against intracellular immune responses that degrade RNA.

In addition, chemical modifications provide greater stability and protection from exonucleases to the guide RNA, which may allow for improved editing of challenging genomic targets and cell types, including plants and prokaryotes.

Essential for Primary and Stem Cells

Highest Editing Efficiency & Reproducibility

Critical for Therapeutic Applications

Improved Editing in Plant and Prokaryotic Cells

Chemical Structure of Synthego Modifications

The chemically modified versions of our synthetic RNA contain 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the 5’ and 3’ terminal three bases of the gRNA.

Chemically modified options can be added to sgRNA, crRNA:tracrRNA and Custom RNA products.

Note: The standard modifications described above are not suited for use with Cpf1-based gRNA. Please contact us if you are interested in specialty modifications that are currently being evaluated for use with Cpf1.

Chemical Structure of Synthego Modifications

Chemically Modified Guide RNA in CD34+ Stem Cells

Collaboration with Stanford University

– CD34+ Human Cord Blood Cells
– RNP Formation
– Transfection: Electroporation
– Editing Efficiency Measured by TIDE Analysis

Chemically Modified Guide RNA in CD34+ Stem Cells

Synthego’s chemically modified sgRNA provides a critical tool for our CRISPR research when it comes to difficult stem cell gene targets. Our research into stem cell-based human therapeutics presents editing challenges that require the highest efficiency guides.

Andrew Scharenberg, MD, Principal Investigator Seattle Children’s Research Institute

[Synthego’s] product allows us to quickly evaluate, in a cost-effective manner, the best guide RNA to stimulate genome editing, including by homologous recombination, in therapeutically relevant human stem cells and primary human T-cells. I highly recommend them.

Matthew Porteus, MD, PhD, Principal Investigator Stanford School of Medicine

Modified 100-mer sgRNA Pricing

Contact Us for Special Academic and Volume Pricing

Modified sgRNA Kit


  • Editing Efficiency +++++
  • Excellent Reproducibility
  • Primary and Stem Cells; Therapeutic Applications
  • Full Length 100-mer Single Guide
  • Your 17-23nt Target Sequence
  • No Annealing
  • Material for 10 to 20 Transfections
  • 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 7-9 Days

Additional quantities are available
Optimized for S. pyogenes Cas9
Plate orders only include RNA; Cas9 and buffers can be added on the Order page.


Poster Download: Improved CRISPR Editing

This poster looks at the use of synthetic sgRNAs, including chemically modified versions, for improved CRISPR editing in various cell types. Download for free to learn more.