ICE CRISPR Analysis Tool

NGS-Quality Editing Analysis With Sanger Sequencing

ANALYZE DATA

Synthego’s Inference of CRISPR Edits (ICE) is a free software tool that offers fast and reliable analysis of Sanger sequencing data. Simply upload your sequencing data into our streamlined web tool to view total insertion and deletion (indel) frequency, relative frequency of each indel, a knockout (KO) score that reveals the proportion of large or out of frame indels likely to generate complete loss-of-function mutations, and total differences between the edited and wild type sequences.

ICE offers accurate results that correlate strongly with next-generation sequencing (NGS)-based amplicon seq, and is compatible with data generated by both single guide and multiplex editing strategies.  This software generates highly accurate analyses without any additional user inputs, making it incredibly easy to use.

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Read “Inference of CRISPR Edits from Sanger Trace Data” on bioRxiv

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Synthego ICE Software Benefits

RESULTS IN UNDER 10 SECONDS
Results comparable to NGS-based amplicon seq in a fraction of the time.

FREE TO USE
ICE is FREE to everyone: no pricing tiers, no services to purchase, ever.

BATCH PROCESS UP TO 700 SAMPLES
Upload files for each batch and rapidly view results.

PUBLICATION-READY FIGURES
High-quality results including Sanger trace, discordance, total indel percentage, and relative frequency of each indel.

Achieve NGS-Quality Results from Sanger Sequencing Data

ICE offers free and accurate analysis of Sanger sequences with NGS quality results, in seconds. Analyze up to 700 samples at a time, download your results with publication-quality figures.

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Comparison of ICE and NGS-based Analyses

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Figure 1. 92 samples were analyzed by both NGS and ICE to evaluate ICE accuracy. NGS-based amplicon seq was performed with 200-300 bp amplicons, while Sanger sequencing used 500-800 bp amplicons. These ICE analysis samples have a good fit with a correlation r2 = 0.96 and ICE samples with poor fit have a correlation r2=0.88.

Figure 2. ICE analysis is comparable to NGS-based analysis for both large and small indels. Green bars are ICE predictions, black bars are NGS-based amplicon seq predictions. ICE provides indel contributions as well as NGS does, at similar percentages. Both ICE and NGS-based amplicon seq are able to find large (>30) deletions and agree at the sequence level

ICE Delivers Publication-Ready Figures

An example of the outputs from the ICE software, demonstrating the relative frequency or contributions of each indel within a population. In this example, a deletion of 25 nucleotides represents 66.2% of the edited genomes within this population.

ICE Outputs Example
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