Figure 1. 92 samples were analyzed by both NGS and ICE to evaluate ICE accuracy. NGS-based amplicon seq was performed with 200-300 bp amplicons, while Sanger sequencing used 500-800 bp amplicons. These ICE analysis samples have a good fit with a correlation r2 = 0.96 and ICE samples with poor fit have a correlation r2=0.88.
Figure 2. ICE analysis is comparable to NGS-based analysis for both large and small indels. Green bars are ICE predictions, black bars are NGS-based amplicon seq predictions. ICE provides indel contributions as well as NGS does, at similar percentages. Both ICE and NGS-based amplicon seq are able to find large (>30) deletions and agree at the sequence level