CRISPRevolution CRISPR/Cas9 EZ RNA Kit

STARTING AT $79

The CRISPR/Cas9 EZ RNA Kit is designed for researchers conducting simple CRISPR genome editing or exploring CRISPR for the first time. The CRISPR kit contains all the necessary components to successfully transfect, target and edit a gene using CRISPR/Cas9 and synthetic guide RNA. Available in two guide RNA formats, sgRNA and cr:tracrRNA, the kit can be purchased with the Cas9 nuclease or without it.

Single Guide RNA Format

No need to anneal, the sgRNA format arrives ready for transfection. It uses a single strand of user-defined 17-20mer RNA target sequence that will bind to the DNA 17-20mer target sequence that is opposite to the PAM sequence.

An optimized 80mer Synthego Scaffold is added on the 3′ end of your target sequence. For example, you will receive a sgRNA with the following format:

5′-AAUUUCACAGCUGCACAUA+Synthego Scaffold-3′

Two-Part cr:tracrRNA Format

crRNA

You provide the 17-20mer RNA sequence that binds to the DNA 17-20mer target sequence that is opposite to the PAM sequence.  An optimized 22mer Linker is added on the 3′ end of your target sequence. For example, you will receive a crRNA with this format:

5′-AAUUUCACAGCUGCACAUA+Synthego Linker-3′

tracrRNA

We have optimized the 72mer tracrRNA sequence based on S. pyogenes, and provide this with a proprietary linker to duplex with the crRNA. You do not need to provide a sequence for EZ tracrRNA.

Need Chemical Modifications?

For a chemically modified version of our Custom RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. Stem Cells, K562, Prokaryotic) and genomic targets that prove otherwise challenging to edit. If you’re interested in a chemically modified option, select Modifications from the dropdown menu when inputting your target sequence on the order page.

Pricing

Contact Us for Special Academic and Volume Pricing

CRISPR/Cas9 EZ RNA Kits

sgRNA

$395

/1 nmol
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10 to 20 Transfections
  • sgRNA (1 nmol)
  • TE Buffer
  • Nuclease-free water
  • Cas9 (300 pmol)
  • Modified RNA Optional*
  • Ships in 5-7 Days

CRISPR RNA Kits (No Cas9)

sgRNA

$295

/1 nmol
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10 to 20 Transfections
  • sgRNA (1 nmol)
  • TE Buffer
  • Nuclease-free water
  • Modified RNA Optional*
  • Ships in 5-7 Days

* Chemically modified options ship in 7-10 days.
Plate orders do not include Cas9 and buffers; Cas9 and buffers can be added on the Order page.

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For general inquiries or questions, give us a call at 888-611-6883 or send us a message

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