CRISPRevolution Synthetic sgRNA Kit


Achieve up to 97% editing efficiency with the highest quality synthetic sgRNA in the market. Synthego is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships up to 4X faster and 80% lower cost.

Benefits of Synthetic sgRNA

No need to anneal crRNA:tracrRNA
Higher efficiency and reduced lab time

Better in vivo stability
When duplexed with Cas9 nuclease

Highly scalable for large numbers of experiments

100% DNA-free
No risk of integrating foreign DNA into cell line

A single oligonucleotide
Ready for transfection out-of-the box

Optimized for SpCas9
Optimized 80mer Synthego scaffold for use with SpCas9

No need to anneal, the sgRNA format arrives ready for transfection. It uses a single strand of user-defined 17-23nt RNA target sequence that will bind to the DNA target sequence that is opposite to the PAM sequence.

An optimized 80mer Synthego Scaffold is added on the 3′ end of your target sequence. For example, you will receive a sgRNA with the following format:

5′-AAUUUCACAGCUGCACAUA+Synthego Scaffold-3′

CRISPRevolution sgRNA Purity vs. IVT

Synthego Synthetic sgRNA

Superior quality leads to consistent editing efficiencies.


Mass-spec trace of synthetic AAVS1 gene 100nt sgRNA (Synthego). Large single peak represents full-length product.

IVT-derived Guide RNA

Increased impurities cause off-target effects.


Mass-spec trace of IVT-derived AAVS1 gene 105nt sgRNA. Large n-1, n+1 and n+2 species are present.

CRISPRevolution sgRNA Efficiency & Consistency

The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.


Looking to Maximize CRISPR Editing in Your Cell Type? Try Chemically Modified Synthetic RNA.

Chemically modified synthetic sgRNAs provide superior CRISPR/Cas9 editing in primary cells, stem cells and challenging cell lines, including cancer cell lines and those of hematopoietic origin. They also provide the highest levels of editing efficiency in most immortalized cell lines, prokaryotes and plants, and are recommended for these applications. Please visit our Chemically Modified RNA page for more information.

For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. Any questions, please contact us.


Contact Us for Special Academic and Volume Pricing

Modified sgRNA Kit


  • Editing Efficiency +++++
  • Excellent Reproducibility
  • Primary and Stem Cells; Therapeutic Applications
  • Full Length 100-mer Single Guide
  • Your 17-23nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 7-9 Days
sgRNA Kit


  • Editing Efficiency ++++
  • Excellent Reproducibility
  • General Cell Types and Basic Applications
  • Full Length 100-mer Single Guide
  • Your 17-23nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 7-9 Days

Additional quantities are available
Optimized for S. pyogenes Cas9
Plate orders only include RNA; Cas9 and buffers can be added on the Order page.


Have a Question? We’d Love to Hear from You.

For general inquiries or questions, give us a call at 888-611-6883 or send us a message