Full Length Synthetic sgRNA


Achieve 90% editing efficiency with the highest quality synthetic sgRNA in the market. Synthego is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships ready for transfection in 5-7 days at 5X lower cost.

Synthego Comp 1 Comp 2
Price $295 $1,500 $1,100
Editing Results +++ ++ ++
Turnaround 5 to 7 Days 2 to 4 Weeks 1 to 2 Weeks
Throughput High Volume Low Volume Low Volume

Contact Us for Special Academic and Volume Pricing

The Benefits of Synthetic sgRNA

CRISPRevolution sgRNA is not only a critical component for CRISPR therapeutics, but also essential for applications where high purity at a reasonable cost is important.

No need to anneal crRNA:tracrRNA
Higher efficiency and reduced lab time

Better in vivo stability
Protection against intracellular exonucleases

100% DNA-free
No risk of integrating foreign DNA into cell line

A single oligonucleotide
Ready for transfection out-of-the box

Highly scalable for large numbers of experiments

sgRNA Mass Spectrometry


This Synthego 100-mer sgRNA was produced using our standard synthesis and processing methods. Data shown is the deconvoluted spectra of a direct injection obtained using an Agilent 6530 Accurate Mass Q-TOF MS.

CRISPRevolution sgRNA Efficiency & Consistency

The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.


Working with Stem Cells or other Difficult Targets? Try Chemically Modified sgRNA.

Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit.

For a chemically modified version of our synthetic sgRNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. If you’re interested in a chemically modified option, select Modifications from the dropdown menu when inputting your target sequence on the order page or contact us.

Synthetic sgRNA

Contact Us for Special Academic and Volume Pricing

CRISPR sgRNA Kit (No Cas9)


/1 nmol
  • Your 17-20nt Target Sequence
  • Material for 10-20 Transfections
  • Modified RNA Optional*
  • Ships in 5-7 Days
Custom RNA 100


/1 nmol
  • Your 76-100nt Sequence
  • Material for 10-20 Transfections
  • Modified RNA Optional*
  • Ships in 5-10 Days

* Ships in 10-15 days.


Have a Question? We’d Love to Hear from You.

For general inquiries or questions, give us a call at 888-611-6883 or send us a message