Full Length Synthetic sgRNA

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Achieve 90% editing efficiency with the highest quality synthetic sgRNA in the market. Synthego is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships ready for transfection in 5-7 days at 5X lower cost.

Synthego Comp 1 Comp 2
Price $295 $1,500 $1,100
Editing Results +++ ++ ++
Turnaround 5 to 7 Days 2 to 4 Weeks 1 to 2 Weeks
Throughput High Volume Low Volume Low Volume

Contact Us for Special Academic and Volume Pricing

Benefits of Synthetic sgRNA

No need to anneal crRNA:tracrRNA
Higher efficiency and reduced lab time

Better in vivo stability
Protection against intracellular exonucleases

Cost-effective
Highly scalable for large numbers of experiments

100% DNA-free
No risk of integrating foreign DNA into cell line

A single oligonucleotide
Ready for transfection out-of-the box

CRISPRevolution sgRNA Purity vs. IVT

Synthego Synthetic sgRNA

Superior quality leads to consistent editing efficiencies.

AAVS1-synthetic-100mer

Mass-spec trace of synthetic AAVS1 gene 100nt sgRNA (Synthego). Large single peak represents full-length product.

IVT-derived Guide

Increased impurities cause off-target effects.

AAVS1-IVT-105mer

Mass-spec trace of IVT-derived AAVS1 gene 105nt sgRNA. Large n-1, n+1 and n+2 species are present.

CRISPRevolution sgRNA Efficiency & Consistency

The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.

sgrna-editing-efficiency-ivt

Working with Stem Cells or other Difficult Targets? Try Chemically Modified sgRNA.

Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit.

For a chemically modified version of our synthetic sgRNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. If you’re interested in a chemically modified option, select Modifications from the dropdown menu when inputting your target sequence on the order page or contact us.

Synthetic sgRNA

Contact Us for Special Academic and Volume Pricing

CRISPR sgRNA Kit (No Cas9)

$295

/1 nmol
  • Your 17-20nt Target Sequence
  • Material for 10-20 Transfections
  • Modified RNA Optional*
  • Ships in 5-7 Days
Custom RNA 100

$429

/1 nmol
  • Your 76-100nt Sequence
  • Material for 10-20 Transfections
  • Modified RNA Optional*
  • Ships in 5-10 Days

* Chemically modified options ship in 7-10 days.

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Have a Question? We’d Love to Hear from You.

For general inquiries or questions, give us a call at 888-611-6883 or send us a message

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