What if there was a way to guarantee efficient CRISPR editing in all human cell types? CRISPR has transformed the field of genome engineering by making it simpler to conduct genetic research. However, it is difficult to consistently achieve high editing efficiency, especially in certain cell types such as primary cells and stem cells.
Various companies have attempted to address the challenges associated with conducting successful CRISPR experiments by offering gene editing kits (see Table 1 below). The efficiency of these kits can vary widely, and there is no guarantee of their results. These other kits also require time-consuming cloning prior to performing the gene editing assays, which often adds weeks to the experimental timelines.