What if there was a way to guarantee efficient CRISPR editing in all human cell types? CRISPR has transformed the field of genome engineering by making it simpler to conduct genetic research. However, it is difficult to consistently achieve high editing efficiency, especially in certain cell types such as primary cells and stem cells.
Various companies have attempted to address the challenges associated with conducting successful CRISPR experiments by offering gene editing kits (see Table 1 below). The efficiency of these kits can vary widely, and there is no guarantee of their results. These other kits also require time-consuming cloning prior to performing the gene editing assays, which often adds weeks to the experimental timelines.
To address these issues, Synthego has recently announced the launch of the industry’s first gene knockout kit with a guaranteed editing efficiency of greater than 50% in any human cell line. This means for the first time, researchers will receive a predictable genome engineering outcome or receive their money back. The CRISPRevolution GKO kit puts high-quality gene editing results within reach of all CRISPR researchers, including those developing emerging therapeutic approaches with increasing genome engineering demands such as CAR-T immunotherapy and in vivo human treatment of monogenic diseases.
How Can Synthego Offer This Guarantee?
An important factor in ensuring the high editing efficiency of Synthego’s GKO kit is the chemically modified synthetic sgRNA. Compared to plasmid-derived guide RNAs, in vitro transcribed guide RNAs, and 2-part cr:tracr RNAs, synthetic sgRNAs have been shown to achieve higher editing efficiency and consistency, while also requiring less lab work. Additionally, chemical modification of sgRNA improves editing efficiencies by improving RNA stability, and is required for efficient editing of many difficult cell types, including primary cells and stem cells. >90% gene editing efficiency can be consistently achieved with Synthego’s GKO kit.
The guide RNA design process is also critical to the success of CRISPR editing experiments. Synthego’s lighting fast and user-friendly CRISPR design tool uses the latest scoring algorithms to find the optimal guide RNA sequences with low predicted off-target effects for each target gene of interest, and the GKO kit can be ordered directly from within the design tool. The kit includes four top-scoring chemically modified sgRNAs, ready for transfection, as well as highly active recombinant Cas9 and positive control reagents.
What Researchers Are Saying About Synthego’s GKO Kit
“A constant challenge for our research is trying to reduce the number of clones we need to screen to find a desired targeted modification. In our tests, the Gene Knockout Kit gave us greater than 80 percent knock-out rates for seven targets,” said Shondra Miller, Ph.D., Director, Center for Advanced Genome Engineering at St. Jude Children’s Research Hospital. “This leads to a dramatic reduction in clonal screening efforts which reduces cost and turnaround times, increasing our bandwidth and ability to do more experiments.”
“Synthego’s kits enable us to rapidly modify difficult-to-edit human hematopoietic stem cells, as well as primary T cells, in a cost-effective manner,” said Daniel Dever, Ph.D., Research Instructor in the Porteus Lab at Stanford University. “Their platform is highly reliable for mediating high-level genome editing in therapeutically relevant cell types and has the potential to accelerate the translation of CRISPR/Cas9-based genome editing to benefit human health.”
What Will You Do With Guaranteed CRISPR Editing?
The GKO is part of Synthego’s CRISPRevolution family of world-class synthetic guide RNA solutions, and includes the industry’s only money-back guarantee of 50 percent or better CRISPR editing efficiency in any human cell line for research use. The CRISPRevolution Gene Knockout Kit is immediately available and ships in as little as seven days.
How will you take advantage of this amazing advancement in CRISPR research?
For more information and a limited time trial offer, please visit synthego.com/knockout.