CRISPR is igniting a revolution. A relatively recent discovery in the timeline of biotechnology, CRISPR is quickly becoming a standard and flexible laboratory tool. Designed to ease any scientist into the world of CRISPR by providing an overview of its fundamentals, this comprehensive guide will help beginners learn the ins and outs of the technique.
Synthetic gRNA offers a new approach that boasts superior editing efficiency, more consistent results, and faster overall workflow. To learn more about how synthetic gRNA compares to the traditional techniques, get your free copy of the Synthetic Guide RNA for CRISPR Genome Editing White Paper AND 5 Things Your PI Should Know About Synthetic RNA.
Are you still using in vitro transcription (IVT) to make your CRISPR guides? While it was once the trusted method for making CRISPR guides, IVT is no longer the most effective way to generate gRNA for CRISPR experiments. This new eBook gives you 3 Reasons to Stop Using IVT and improve your CRISPR experiment.
Download this free eBook to learn How to Perform Successful CRISPR Experiments. This Step-by-Step guide includes a simple and easy-to-follow experimental workflow as you embark on your CRISPR experiment journey. In addition, we include recommendations regarding CRISPR components, delivery techniques and analysis methods.
Studies show that a ribonucleoprotein (RNP) complex between Cas9 nuclease and synthetic sgRNA produce the most consistent genome edits with the lowest off-target effects and no risk of DNA incorporation compared to plasmid or mRNA-based approaches. Download this collaboration poster to see the editing results from multiple cell types.
In this on-demand webinar, we review and analyze the results of the “State of CRISPR” survey and look at predicted CRISPR trends of 2017. Download your free recording to learn more about current gene editing trends, CRISPR applications, CRISPR Guide RNAs and challenges facing CRISPR researchers.
In this on-demand webinar, you will learn how to get started with a successful CRISPR experiment, and understand the advantages of synthetic RNA compared to plasmid and IVT approaches. You’ll see the results of CRISPR editing from collaborators and researchers who have been working with Synthego guide RNA products.
The Protocols Pack (Lipofection, Nucleofection, Electroporation) describes how to transfect cultured cells with ribonucleoprotein (RNP) complexes that consist of purified Cas9 nuclease duplexed with synthetic guide RNA (gRNA; synthetic sgRNA or annealed crRNA and tracrRNA). Combined with high-quality synthetic guide RNA, you’ll be able to achieve unparalleled consistency between your CRISPR experimental replicates.