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PRODUCT

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit

Real-time fluorescent RT-RPA for RNA targets (exo)

Integrates reverse transcription with endpoint RPA for fast RNA detection at low constant temperatures. Lyophilized 2× mix simplifies setup for gel or lateral‑flow analysis in ~10–20 minutes.

  • RT integrated for RNA targets
  • Rapid results in ~10–20 minutes
  • 37–42 °C low‑temperature detection
  • Endpoint readout via gel or lateral flow

Select a Size

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit (8 rxn)

#MM32RTRPAEXO-Sm

Product Specifications

Product Number MM32RTRPAEXO
Function RT-RPA with exo probes for real-time fluorescence
For Use In Rapid RNA target quantification
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Sensitive and Specific RNA Detection in Real Time

The RT-RPA Real-Time Kit is a ready-to-use, high-performance solution for real-time RNA amplification under isothermal conditions. Operating at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific RNA detection in as little as 10 to 20 minutes. Its lyophilized format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Powered by a proprietary reverse transcriptase optimized for isothermal amplification, the kit ensures accurate and efficient cDNA synthesis within the reaction. It is carefully formulated for fluorescence-based real-time detection, delivering strong signal clarity and enabling real-time monitoring of amplification with minimal hands-on time. Its robust performance across diverse sample types, including crude extracts, makes it a versatile tool for time-critical diagnostics and research applications.

Key Features

  • Ready-to-Use Lyophilized Format: Stable for ambient storage and transport, eliminating the need for refrigeration.
  • Ultra-Rapid Amplification: Achieves sensitive RNA detection in just 10–20 minutes.
  • Proprietary Reverse Transcriptase: Engineered for efficient cDNA synthesis at isothermal temperatures.
  • Real-Time Monitoring: Optimized for fluorescence-based detection with strong signal clarity.
  • Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.

Applications

  • Pathogen Detection: Rapid and reliable RNA amplification for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants in field-based settings.
  • Assay Development: Suitable for research and development of diagnostic assays.
  • Flexible Readouts: Compatible with real-time fluorescence, lateral flow, and other detection methods.

Custom Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Functional Assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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