PRODUCT

T4 RNA Ligase II

High-specificity ligase for RNA end-joining

T4 RNA Ligase II ligates 3′‑OH RNA to 5′‑phosphorylated oligos in a duplex context, suppressing adapter‑dimers and artifacts. It enables cleaner small RNA libraries and supports dsRNA nick sealing and RNA circularization across scales.

Duplex‑guided ligation suppresses adapter‑dimers
Cleaner small RNA libraries and profiles
Supports dsRNA nick sealing and circularization
Scales from pilot to high‑throughput workflows

Select a Size

T4 RNA Ligase II (1000 U)

#R34T4RNALIG-Sm

Specifications Description Quality Resources

Product Number	R34T4RNALIG
Intended Use	This product is for research use only
Function	Joins 3′-OH RNA to 5′-phosphorylated oligos in duplex context
For Use In	Small RNA-Seq 3′ adapter ligation, RNA circularization, dsRNA nick repair
Source	Recombinant E. coli
Concentration	50 U/µL
Purity	≥ 95%
Storage	-20 C
Stability	24 months
Shipping	Dry Ice
Heat Inactivation	Not recommended, may denature dsRNA; terminate with Proteinase K or EDTA
Delivery Format	Tubes

Description

High-Specificity Ligase for RNA End-Joining

T4 RNA Ligase II is an ATP-dependent RNA ligase that catalyzes the formation of phosphodiester bonds, enabling both inter- and intramolecular RNA strand joining. This enzyme is specifically optimized for joining nicks on double-stranded RNA (dsRNA) and is significantly more active in this function compared to T4 RNA Ligase I. Additionally, it can ligate the 3´-OH of RNA to the 5´-phosphate of DNA in a double-stranded structure, making it a versatile tool for RNA and DNA manipulation.

Key Features

ATP-Dependent Activity: Catalyzes RNA strand joining via phosphodiester bond formation.
High Specificity for dsRNA: Superior activity for joining nicks on double-stranded RNA compared to single-stranded RNA.
Versatile Ligation: Capable of ligating the 3´-OH of RNA to the 5´-phosphate of DNA in double-stranded structures.
Precise Requirements: Requires an adjacent 5´-phosphate and 3´-OH for ligation.

Applications

Joining Nicks on Double-Stranded RNA: Ideal for repairing or modifying dsRNA molecules.
RNA-DNA Hybrid Ligation: Joins the 5´-phosphate of oligodeoxyribonucleotides to the 3´-OH of oligoribonucleotides.
Rapid Amplification of cDNA Ends: Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5´-RACE

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

One unit is defined as the amount of enzyme required to ligate 0.4 μg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction of 20 μl in 30 minutes at 37°C.

Purity (Bis-Tris PAGE): ≥95.0%

Purity (SEC-HPLC): ≥95.0%

Activity: ≥50 U/μl

Residual RNase: Negative in T4 RNA Ligase II; Negative in 10X T4 RNl2 Reaction Buffer)

RESOURCES