Early discovery breakthroughs in therapeutic development begin with research single guide RNA (sgRNA).
Synthego’s research-grade sgRNA delivers high-quality, synthetic guides engineered for consistent performance across novel, engineered, and well-established nucleases.
Having synthesized over 1 million sgRNAs with up to 99% editing efficiency, we give researchers the performance, customization, and scalability needed at every step to move from early discovery to clinical development with confidence.
When it's time to advance to preclinical and clinical work, our true cGMP sgRNA provide the regulatory-grade quality required for submission-ready programs. Partner with a manufacturer equipped to support you throughout the entire journey.
Broad Nuclease Compatibility:
Engineered for use with a wide range of CRISPR nucleases to support efficient editing across research applications.
Flexible Chemical Modifications:
Available as standard, custom, and unmodified formats, enabling you to tailor sgRNA stability and performance to your experimental needs.
Scalable Production:
Offered in multiple lengths, yields, and purification options to support projects from early discovery to advanced research studies.
| Pricing Starting At: |
|
| Deliverables: |
|
| Yield Offerings: |
|
| gRNA Length: |
|
| Modifications: |
|
| QC and Purification: |
|
To design your own guide RNA, select your target sequences using your favorite CRISPR design platform, such as CHOPCHOP, Benchling, CRISPOR, or Cas-Designer CRISPR RGEN Tools.
PAM sequences for eSpOT-ON, hfCas12Max, AccuBase, and SpCas9 are below:
To order your guide RNA, upload your target sequences without the PAM sequence into our easy-to-use ordering interface so we can synthesize your guide RNA.
SpCas9 nuclease is widely used to perform CRISPR experiments. When complexed with our sgRNA, SpCas9 achieves editing efficiencies that are unattainable with other sgRNA options.
Not all cell types are easy to perform CRISPR experiments in. Certain cell types, like human resting T cells, can be challenging to achieve high editing efficiencies while maintaining high cell viability.
Not all chemically synthesized sgRNA are manufactured equally. During synthesis, some oligomers fail to extend during each cycle resulting in undesired truncated molecules mixed in with your desired sgRNA. Additionally, several types of chemical contaminants can be introduced through the manufacturing process of sgRNA. The presence of both these impurities can increase off-target binding, reduce on-target binding, or both. Several vendors offer high-performance liquid chromatography (HPLC) as a purification step to eliminate the unwanted truncated oligomers (n-3 to n-5) and contaminants. However, each vendor might have different synthesis and quality standards to reach the purity levels desired for your sgRNA.
Synthego’s best-in-class sgRNA can achieve exceptional editing efficiencies through standard manufacturing processes (non-HPLC) compared to HPLC purified sgRNA from other vendors. If the purity was substantially better, you would expect the HPLC purified sgRNA to perform better in all cases. However, that is not always the case in our study.
Depending on your application, particularly sensitive applications like therapeutic development require sgRNA that achieves high performance in early development and highly pure sgRNA as development progresses towards the clinic.
If you have additional questions please connect with a member of our team.
Synthego Research sgRNAs are designed to be compatible with common CRISPR nucleases, including SpCas9, Cas12a, and many engineered high-fidelity or enhanced-specificity variants. Different sgRNA sequences and chemical modifications are tailored to the requirements of each nuclease. When selecting a gRNA, it’s important to ensure that the guide sequence and scaffold are matched to the nuclease you plan to use, as factors like PAM compatibility, cut pattern, and nuclease-specific structure can impact editing efficiency and specificity.
Mid-range yields (10–30 nmol) support multi-condition optimization or extended editing workflows.
High yields (50–100+ nmol) are ideal for large-scale cell engineering, replicates, or pooled projects.
-Require maximum purity for sensitive applications
-Performing high-precision knock-ins
-Working with challenging delivery conditions
See our sgRNA Purity without HPLC resource for detailed data.
Higher editing efficiency: increased stability improves the window of active nuclease-guide complex formation.
Reduced innate immune response: modified guides limit activation of cellular immune sensors. This is critical for primary cells and therapeutic workflows.
Improved consistency: chemical stabilization reduces variability between experiments and helps maintain performance across different cell types.
Cart
Account
Your Orders
Sign In