PRODUCT

Pyrophosphatase, Inorganic

Boost mRNA yield by removing pyrophosphate

In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions.

Maximize IVT yield by removing PPi product inhibition
Improve full‑length purity and reduce truncated RNA
Simple spike‑in; no buffer changes to your mix
Cost‑efficient: more mRNA per run saves template and NTPs

Pyrophosphatase, Inorganic

#R25PYROPHOS-Med

Specifications Description Quality Resources

Product Number	R25PYROPHOS
Function	Hydrolyzes pyrophosphate to phosphate; relieves product inhibition
For Use In	in vitro transcription (IVT) yield enhancement, RNA synthesis optimization
Concentration	0.1 U/µL
Molecular Weight	33.4 kD
Source	Recombinant E. coli
Shipping Conditions	Dry Ice
Storage Temperature	-20 C
Purity	≥ 95%
Delivery Format	Tubes
Intended Use	This product is for research use only

Description

Efficient Pyrophosphate Hydrolysis

Pyrophosphatase, Inorganic is a high-quality enzyme designed to hydrolyze inorganic pyrophosphate generated during in vitro transcription (IVT) reactions. By removing unwanted pyrophosphate, the enzyme shifts the reaction equilibrium toward RNA synthesis, significantly increasing RNA yield. This makes it an essential tool for mRNA production in both research and therapeutic applications.

Key Features

Enhances RNA Yield: Hydrolyzes pyrophosphate to shift equilibrium toward RNA synthesis.
Safe and Reliable Antibiotic-free and animal-free production for therapeutic applications.
Optimal Conditions: Requires Mg²⁺ as a cofactor and performs best at pH 7.0.

Applications

Essential for In Vitro Transcription (IVT): Boost RNA yield by removing pyrophosphate during transcription.
mRNA Therapeutics and Vaccine Manufacturing: Ideal for large-scale production of high-quality mRNA.

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

One unit is defined as the amount of enzyme that will generate 1 μmol of orthophosphate per minute from inorganic pyrophosphate (PPi) in a 10 minute standard reaction at 25°C containing 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, and 2 mM PPi.

Purity (SEC-HPLC): ≥95.0%

Endotoxin: ≤10.0 EU/ml

Residual RNase: Negative

Activity (Enzyme Catalytic Efficiency): ≥0.1 U/μl

RESOURCES