PRODUCT

RNase R

High-specificity enzyme for targeted RNA depletion

RNase R is a 3′→5′ exoribonuclease that degrades linear RNAs while sparing circular and structured species. Enrich specialized RNA populations and sharpen transcriptome analyses without losing circRNAs or lariat structures.

Depletes linear RNA; enriches circular/structured forms
Enhances circRNA and non‑coding RNA studies
Improves clarity in transcriptome analyses
Scales from targeted tests to HT pipelines

Select a Size

RNase R (2000 U)

#R34RNASER-Sm

Specifications Description Quality Resources

Product Number	R34RNASER
Function	3′ to 5′ exoribonuclease that digests linear RNA, spares circular and lariat
For Use In	circRNA enrichment, non-coding RNA research, RNA QC and depletion workflows
Concentration	20 U/µL
Molecular Weight	92 kDa
Source	Recombinant E. coli
Shipping Conditions	Dry Ice
Storage Temperature	-20 C
Stability	24 Months
Purity	≥ 95%
Delivery Format	Tubes
Intended Use	This product is for research use only

Description

High-Quality RNA Degradation Enzyme

RNase R is a highly efficient exoribonuclease derived from E. coli that digests linear RNA in a 3’-to-5’ direction, while leaving lariat RNA, circular RNA, and certain structured RNAs intact. This unique specificity makes RNase R an essential tool for studying alternative splicing, gene expression, and RNA structure.

Unlike other RNases, RNase R does not degrade circular RNA, lariat RNA, or double-stranded RNA with short 3’ overhangs (<7 nucleotides). Most cellular RNAs, including mRNA, rRNA, and other linear RNA species, are completely digested, with the exception of tRNAs, 5S RNA, and intron lariats.

Key Features

Selective Digestion: Efficiently digests linear RNA while preserving lariat and circular RNA.
High Specificity: Does not affect double-stranded RNA with short 3’ overhangs (<7 nucleotides).
Versatile Applications: Ideal for isolating circular or lariat RNA from total RNA mixtures.

Applications

Alternative Splicing and Gene Expression Studies: Isolate and analyze circular or lariat RNA to study splicing mechanisms.
Intron cDNA Production: Generate cDNA from intronic RNA for downstream applications.
Intronic Screening of cDNA Libraries: Screen cDNA libraries for intronic sequences with precision.

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.