PRODUCT

DNase I

RNase-free DNase I clears DNA from RNA workflows

RNase‑free DNase I rapidly digests plasmid templates after IVT and removes genomic DNA from RNA preps. Protect RNA integrity and prevent false positives in RT‑qPCR and RNA‑seq with a high‑activity enzyme compatible with common buffers.

Rapid post‑IVT template removal
Clear gDNA for accurate RT‑qPCR and RNA‑seq
High activity across common buffers and kits
RNase‑free: preserves RNA integrity

Select a Size

DNase I (1000 U)

#R20DNASEI-Sm

Specifications Description Quality Resources

Product Number	R20DNASEI
Function	Endonuclease cleaving ssDNA and dsDNA into oligonucleotides
For Use In	DNA removal from IVT mRNA prep, genomic DNA clearance from RNA isolations
Concentration	4 U/µL
Source	Recombinant E. coli
Shipping Conditions	Dry Ice
Storage Temperature	-20 C
Stability	24 Months
Purity	≥ 95%
Heat Inactivation	10 min at 65 C
Delivery Format	Tubes
Intended Use	This product is for research use only

Description

High-Purity DNase for DNA Degradation

DNase I is a versatile endonuclease that efficiently digests both single- and double-stranded DNA into oligonucleotides with 5´-phosphate and 3´-hydroxylated ends. This enzyme is essential for applications requiring precise DNA removal, ensuring high-quality RNA samples and reliable downstream results.

DNase I is widely used in RNA purification workflows, where it eliminates contaminating DNA to improve the accuracy of RNA-based applications. It is also ideal for in vitro transcription (IVT) processes and DNase I footprinting, making it a valuable tool for molecular diagnostics and mRNA synthesis.

Key Features

Broad Substrate Range: Efficiently degrades both ssDNA and dsDNA.
Optimized Activity: Performs effectively across a wide range of conditions and buffers.
Versatile Applications: Ideal for DNA removal in RNA prep, protein purification, and cell lysate treatments.

Applications

In Vitro Transcription: Ensures DNA-free RNA templates for efficient mRNA synthesis.
RNA Sample Preparation: Removes DNA contamination from RNA samples for high-purity results.
DNase I Footprinting: Maps protein-DNA interactions with precision.

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.