PRODUCT

Proteinase K, lyophilized

Protease for nuclease inactivation and cleanup

Broad‑spectrum serine protease rapidly inactivates RNases/DNases and digests proteins. Stable lyophilizate with PCR‑grade purity supports DNA/RNA extraction and cleanup across varied samples and conditions.

Rapid RNase/DNase inactivation
Efficient protein digestion and cleanup
Stable lyophilizate; PCR‑grade purity
Works across varied samples and buffers

Select a Size

Proteinase K, lyophilized (30 mg)

#SP33PROTK-Sm

Specifications Description Quality Resources

Product Number	SP33PROTK
Function	Serine protease active across broad conditions
For Use In	DNA/RNA extraction and clean up
Source	Recombinant E. coli
Shipping Conditions	Ambient
Storage Temperature	Ambient
Stability	≥12 months
Delivery Format	Tubes
Intended Use	This product is for research use only

Description

Proteinase K for Protein Digestion

Proteinase K is a highly active endopeptidase designed for nonspecific protein degradation and nucleic acid purification in the most sensitive applications. With exceptional purity and PCR-grade quality, Proteinase K ensures reliable performance in workflows requiring the removal of proteins and the inactivation of nucleases, making it ideal for preparing high-quality PCR templates.

This robust enzyme is stable over a broad pH range (4–12.5) and retains full activity for several hours at pH 6.5–9.5. Its activity is further enhanced by denaturing agents like SDS and urea, with SDS increasing activity up to seven-fold. Proteinase K is available as a stable lyophilized powder or an aqueous solution, offering flexibility for various experimental needs.

Key Features

Exceptional Purity: Free of RNases, DNases, and DNA, ensuring suitability for the most sensitive applications.
PCR-Grade Quality: Optimized for nucleic acid purification and preparation of PCR templates.
Stable Lyophilizate: Long shelf life and reliable performance in diverse workflows.
Broad pH Stability: Active across pH 4–12.5, with optimal activity at pH 6.5–9.5.

Applications

Nonspecific Protein Degradation: Efficiently degrades native proteins for downstream applications.
Nucleic Acid Purification: Removes proteins and inactivates nucleases for high-quality RNA and DNA preparation.
Deactivation of Nucleases: Ensures enzymatic reactions are free from RNase and DNase contamination.

Inhibitors and Activators

Inhibitors: Proteinase K is resistant to inactivation by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, and protease inhibitors like trypsin and chymotrypsin inhibitors.
Activators: Activity is stimulated by denaturing agents such as SDS and urea, with SDS increasing activity up to seven-fold.

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Protein Concentration
The protein concentration of soluble Proteinase K is analyzed by Biuret assay (20 mg, +/- 2 mg).

Protease Activity
The activity is analyzed with hemoglobin as substrate at 37°C (>600 U/ml).

Absence of nuclease activity
The enzyme is tested for absence of endonuclease and nicking activities.

RNase contamination
The enzyme is tested for absence of RNase activity.

DNA contamination
The enzyme is tested for DNA (threshold 10 pg/mg).

Bioburden
The enzyme is tested for biodurden (<1 CFU/ml).

RESOURCES

Download the User Guide

Proteinase K User Guide

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