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PRODUCT

dNTP Mixes

Ready-to-use balanced dNTP mix for routine PCR

Equimolar A/C/G/T at 25 mM each simplifies setup and improves run‑to‑run consistency. Ideal for routine and high‑throughput PCR, library construction, and automated pipelines requiring reproducibility.

  • Equimolar A/C/G/T at 25 mM each
  • Simplifies setup and reduces variability
  • Great for routine and HT PCR
  • Automation‑friendly performance

Select a Size

dNTP Mixes (1 mL)

#N05DNTPMIX-Sm

Product Specifications

Product Number N05DNTPMIX
Intended Use This product is for research use only
Function DNA polymerase substrates in equimolar mix
For Use In Routine PCR, library construction, HT PCR
Concentration 25 mM
Shipping Conditions Cold Pack
Storage Temperature -20 C
Stability ≥12 months
Purity >99%
Delivery Format Tubes
Description

Deoxynucleotide Triphosphates Mixes

dNTP Mixes are high-quality deoxynucleotide triphosphates designed to deliver exceptional performance and reliability across a wide range of PCR and molecular biology applications. With a purity of >99% confirmed by HPLC, these mixes are free from DNA and PCR inhibitors, ensuring consistent and accurate results even in the most demanding workflows.

The advanced production process guarantees outstanding stability, making dNTP Mixes the first choice for applications such as long-range PCR (up to 40 kb), GC-rich template amplification, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling, and sequencing. These ready-to-use mixes simplify reaction setup and ensure optimal performance in every experiment.

Key Features

  • Exceptional Purity: >99% purity confirmed by HPLC for reliable and reproducible results.
  • Inhibitor-Free: Free from DNA and PCR inhibitors to ensure optimal performance.
  • Outstanding Stability: Provides consistent results across standard and demanding PCR applications.
  • Ready-to-Use: Pre-mixed for convenience and flexibility in PCR workflows.

Applications

  • Standard and Hot-Start PCR: Reliable amplification for routine and advanced workflows.
  • Long-Range and High-Fidelity PCR: Amplify long targets (up to 40 kb) and achieve high accuracy.
  • cDNA Synthesis and RT-PCR: Ideal for RNA-based workflows.
  • qPCR: Ensures precise and reproducible quantification.
  • Sequencing and DNA Labeling: High-quality nucleotides for downstream applications.

Usage Recommendations

  • Use 0.2 mM of each nucleotide as the recommended final concentration for common PCR applications.
  • Thaw and mix well before use, especially for highly concentrated solutions.
  • Use dNTP Mixes directly in PCR reactions for convenience and consistent results.

Custom Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.
  • Master Mix Creation: Custom manufacturing of PCR master mixes based on your optimized formulations.

Quality

Functional assay
Functionality tested in PCR.

Purity test
The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test
The absence of human and E. coli DNA is confirmed by qPCR.

RESOURCES

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