Learn more Webcast featuring eSpOT-ON
Explore More eSpOT-ON Nuclease Protein Available Now
Explore More Order eSpOT-ON Nuclease mRNA Now
My Cart
Contact Sales
PRODUCT

dNTP Sets

HPLC-purified dNTPs for PCR, qPCR, and cloning

HPLC‑purified nucleotides supplied individually at 100 mM for A/C/G/T. Mix and match for optimal performance and balanced incorporation in PCR, qPCR, RT‑PCR, and cloning workflows across instruments.

  • ≥99% Purity by HPLC for each nucleotide
  • Supplied individually at 100 mM (A/C/G/T)
  • Flexible formulation for diverse workflows
  • Compatible with PCR, qPCR, RT‑PCR, cloning

Select a Size

dNTP Sets (4 x 250 µL)

#N05DNTPSET-Sm
Product image 1
Specifications Description Quality Resources

Product Specifications

Product Number N05DNTPSET
Intended Use This product is for research use only
Function DNA polymerase substrates (supplied individually)
For Use In PCR, qPCR, RT-PCR, cloning
Concentration 100 mM
Shipping Conditions Cold Pack
Storage Temperature -20 C
Stability ≥12 months
Purity >99%
Delivery Format Tubes
Description

High-Purity, Individual NTPs

dNTP Sets are exceptional-quality deoxynucleotide triphosphates designed to deliver outstanding performance and reliability across a wide range of PCR and molecular biology applications. With a purity of >99% confirmed by HPLC, these dNTPs are free from DNA and PCR inhibitors, ensuring consistent and accurate results even in the most demanding workflows.

Their high quality guarantees outstanding stability, making these dNTPs the first choice for applications such as long-range PCR (up to 40 kb), GC-rich template amplification, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling, and sequencing. For maximum flexibility, dNTPs are available in individual sets, allowing researchers to tailor their experiments with ease.

Key Features

  • Exceptional Purity: >99% purity confirmed by HPLC for reliable and reproducible results.
  • Inhibitor-Free: Free from DNA and PCR inhibitors to ensure optimal performance.
  • Outstanding Stability: Provides consistent results across standard and demanding PCR applications.

Applications

  • Standard and Hot-Start PCR: Reliable amplification for routine and advanced workflows.
  • Long-Range and High-Fidelity PCR: Amplify long targets (up to 40 kb) and achieve high accuracy.
  • cDNA Synthesis and RT-PCR: Ideal for RNA-based workflows.
  • qPCR: Ensures precise and reproducible quantification.
  • Sequencing and DNA Labeling: High-quality nucleotides for downstream applications.

Usage Recommendations

  • Use 0.2 mM of each nucleotide as the recommended final concentration for common PCR applications.
  • Ensure equal concentrations of all four dNTPs in the PCR mixture for optimal results.

Custom Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.
  • Master Mix Creation: Custom manufacturing of PCR master mixes based on your optimized formulations.

Quality

Functional assay
Functionality tested in PCR.

Purity test
The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test
The absence of human and E. coli DNA is confirmed by qPCR.

Ready to get started?

Get in touch