PRODUCT

dUTP

High-purity dUTP for UNG-based carryover control

High‑Purity dUTP suitable for incorporation by many DNA polymerases. Pair with UNG/UDG to minimize carryover contamination in PCR/qPCR. Also useful for labeling and nick translation workflows.

Supports UNG/UDG carryover prevention
Polymerase‑compatible incorporation of dU
Useful for labeling and nick translation
Balances performance and contamination control

dUTP

#N05DUTPSET-Med

Specifications Description Quality Resources

Product Number	N05DUTPSE
Intended Use	This product is for research use only
Function	Uracil Nucleotide Substrate
For Use In	PCR and qPCR with UNG, Nick Translation, Labeling
Concentration	100 mM
Shipping	Cold Pack
Storage Temperature	-20 C
Stability	≥12 months
Purity	>99%
Delivery Format	Tubes

Description

High-Purity, Individual dUTP

High-purity dUTP is designed to deliver outstanding performance and reliability across a wide range of PCR and molecular biology applications. With a purity of >99% confirmed by HPLC, dUTP is free from DNA and PCR inhibitors, ensuring consistent and accurate results even in the most demanding workflows.

Advanced production technology guarantees outstanding stability, making high-purity dUTP the first choice for applications such as long targets, qPCR and cDNA synthesis, high-fidelity PCR, DNA labeling, and sequencing. For maximum flexibility, all our dNTPs are available in sets and mixes at common concentrations, allowing researchers to tailor their experiments with ease.

Key Features

Exceptional Purity: >99% purity confirmed by HPLC for reliable and reproducible results.
Inhibitor-Free: Free from DNA and PCR inhibitors to ensure optimal performance.
Outstanding Stability: Provides consistent results across standard and demanding PCR applications.

Applications

Preventing PCR Contamination: Using dUTP in nucleotide mixes for PCR and qPCR together with UDG/UNG can efficiently prevent carry-over contamination from previous amplification.
Standard and Hot-Start PCR: Reliable amplification for routine and advanced workflows.
Long-Range and High-Fidelity PCR: Amplify long targets (up to 40 kb) and achieve high accuracy.
cDNA Synthesis and RT-PCR: Ideal for RNA-based workflows.
qPCR: Ensures precise and reproducible quantification.
Sequencing and DNA Labeling: High-quality nucleotides for downstream applications.

Custom Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.
Master Mix Creation: Custom manufacturing of PCR master mixes based on your optimized formulations.

Quality

Functional assay
Functionality tested in PCR.

Purity test
The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test
The absence of human and E. coli DNA is confirmed by qPCR.