Robust thermostable polymerase for routine PCR
Standard 5′→3′ DNA polymerase with low 5′→3′ exonuclease and no proofreading. Adds 3′‑A overhangs for TA cloning. Reliable amplification across typical templates and buffers for everyday PCR and genotyping.
| Product Number | EM15TAQDNA |
| Intended Use | This product is for research use only |
| Function | 5' to 3' polymerase; low 5' to 3' exonuclease; no proofreading |
| For Use In | Routine PCR, genotyping, colony PCR, TA cloning |
| Concentration | 5 U/µL |
| Molecular Weight | 94 kDa |
| Source | Recombinant E. coli |
| Storage Temperature | -20 C |
| Shipping Conditions | Cold Pack |
Taq DNA Polymerase is a thermostable enzyme originally derived from the thermophilic bacterium Thermus aquaticus. It catalyzes the 5' to 3' synthesis of DNA and is widely recognized for its robust performance in a variety of PCR applications. Known for its robust performance and versatility, Taq DNA Polymerase is a trusted choice for routine PCR, RT-PCR, and TA cloning workflows. Its ability to deliver high product yields from a variety of templates, combined with its compatibility with both standard and fast PCR protocols, makes it an essential tool for molecular diagnostics and research laboratories.
We offer flexible customization to meet your specific needs:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 72°C in the presence of the reaction buffer.
Functional assay
Human genomic DNA was amplified using the DNA Polymerase and specific primers to produce a distinct band of 750 bp.
Self-priming activity
Standard PCR is carried out without primers, using the DNA Polymerase and human genomic DNA. No products were amplified.
Exonuclease assay
Linearized lambda/HindII fragments are incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No degradation of DNA was observed.
Endonuclease assay
lambda DNA is incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No degradation of DNA was observed.
Nick Activity
Supercoiled plasmid DNA is incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No conversion of covalently closed circular DNA to nicked DNA was detected.
E. coli DNA contamination assay
A sample of the denatured DNA Polymerase is analyzed with specific primers targeting the 16S rRNA gene in qPCR for the presence of contaminating E. coli DNA. No E. coli DNA was detectable.
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