Thermolabile anti-Taq antibody for hot-start PCR
Monoclonal antibody reversibly inhibits Taq at ambient temperature and releases during initial denaturation. Reduces primer‑dimers and mis‑priming for cleaner, more specific amplification setups.
| Product Number | AB27ANTITAQ |
| Intended Use | This product is for research use only |
| Function | Binds and inhibits Taq at RT; Releases on denaturation |
| For Use In | Hot-Start PCR, Multiplex PCR, Assay Development |
| Concentration | 1 mg/mL |
| Class | Monoclonal |
| Source | Cell Culture |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20 C |
Taq DNA Polymerase Antibody is an ultra-pure monoclonal antibody specifically designed to inhibit Taq DNA polymerase activity at ambient temperatures, enabling a convenient and effective hot-start PCR setup. Produced in cell culture to ensure the highest quality, this antibody provides thermolabile inhibition, blocking over 95% of Taq activity at 60°C. It enhances PCR specificity and sensitivity by preventing the formation of primer–dimers and nonspecific amplification during reaction setup.
During the first denaturation step of thermal cycling, the antibody becomes nonfunctional, releasing the active Taq DNA polymerase for efficient DNA amplification. This antibody-mediated hot-start method is faster and more convenient than chemically inhibited polymerases, allowing for room-temperature reaction assembly and rapid polymerase activation.
To create a hot-start Taq DNA polymerase pre-mix:
We offer flexible customization to meet your specific needs:
One unit is defined as the amount of antibody required to inhibit >95% of 1 unit Taq DNA polymerase at 60°C. One unit Taq Antibody (200 ng of the antibody) will ensure inhibition of Taq DNA polymerase.
Functional Assay
Taq Antibody was functionally tested on hot-start PCR.
Inhibition activity
The Taq DNA polymerase activity was inhibited to >95% at 60°C when adding 200 ng Taq Antibody (1 U) to one unit Taq DNA polymerase.
Mouse genomic DNA contamination assay
A Taq Antibody sample is analyzed for the presence of contaminating mouse genomic DNA. No mouse genomic DNA was detectable.
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