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PRODUCT

Recombinase Polymerase Amplification (RPA) Real-Time Kit

Real-time fluorescence-enabled RPA (exo probes)

2× lyophilized master mix supports real‑time detection via exo probes at 37–42 °C with minimal hands‑on time. Ideal for rapid DNA target quantification and workflow integration with standard instruments.

  • Exo‑probe real‑time fluorescence
  • Minimal hands‑on time at 37–42 °C
  • Lyophilized 2× mix; easy integration
  • Ideal for rapid DNA target quantification

Select a Size

Recombinase Polymerase Amplification (RPA) Real-Time Kit (8 rxn)

#MM32RPAEXO-Sm

Product Specifications

Product Number MM32RPAEXO
Function RPA with exonuclease and fluorescent probes
For Use In Real-Time DNA target quantification
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Real-Time DNA Amplification with Fluorescence Monitoring

The RPA Real-Time Kit is a ready-to-use, high-performance solution for real-time DNA amplification under isothermal conditions. Operating at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific DNA detection in as little as 10 to 20 minutes. Its lyophilized format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Optimized for fluorescence-based detection, the kit allows for real-time monitoring of amplification with strong signal clarity and minimal hands-on time. Its robust formulation ensures efficient target recognition and consistent performance, even with complex or unprocessed sample types, making it a reliable choice for time-critical diagnostics and research applications.

Key Features

  • Ready-to-Use Lyophilized Format: Stable for ambient storage and transport, eliminating the need for refrigeration-perfect for point-of-care and remote settings.
  • Ultra-Rapid Amplification: Achieves sensitive DNA detection in just 10–20 minutes.
  • Real-Time Monitoring: Optimized for fluorescence-based detection with strong signal clarity.
  • Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.
  • Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.

Applications

  • Pathogen Detection: Rapid and reliable amplification for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants in field-based settings.
  • Assay Development: Suitable for research and development of diagnostic assays.
  • Flexible Readouts: Compatible with real-time fluorescence, lateral flow, and other detection methods.

Custom Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Functional assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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