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PRODUCT

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Endpoint RNA Detection Kit

Rapid isothermal RNA detection (RT-integrated)

Integrates reverse transcription with endpoint RPA for fast RNA detection at low constant temperatures. Lyophilized 2× mix simplifies setup for gel or lateral‑flow analysis in ~10–20 minutes.

  • RT integrated for RNA targets
  • Rapid results in ~10–20 minutes
  • 37–42 °C low‑temperature detection
  • Endpoint readout via gel or lateral flow

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Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Endpoint RNA Detection Kit (8 rxn)

#MM32RTRPABASE-Sm

Product Specifications

Product Number MM32RTRPABASE
Function RT-RPA with exo probes for real-time fluorescence
For Use In Rapid RNA target quantification
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Fast RNA Detection at Low Temperatures

The RT-RPA Endpoint Kit is a ready-to-use, high-performance solution for ultra-rapid RNA amplification under isothermal conditions. Designed to operate at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific RNA detection in as little as 10 to 20 minutes. Its lyophilized format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Powered by a proprietary reverse transcriptase optimized for isothermal amplification, the kit ensures accurate and efficient cDNA synthesis within the reaction. It is carefully formulated for endpoint detection with compatibility for lateral flow and electrophoresis readouts, delivering clear and reliable results with minimal hands-on time. Its robust performance across diverse sample types, including crude extracts, makes it a versatile tool for time-critical diagnostics and research applications.

Key Features

  • Ready-to-Use Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration.
  • Ultra-Rapid Amplification: Achieves highly sensitive RNA detection in just 10–20 minutes.
  • Proprietary Reverse Transcriptase: Engineered for efficient cDNA synthesis at isothermal temperatures.
  • Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.
  • Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
  • Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

  • Pathogen Detection: Rapid and reliable low-copy RNA amplification for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
  • Assay Development: Scalable RNA amplification from research to high-throughput workflows.
  • Flexible Readouts: Compatible with lateral flow, electrophoresis, and other endpoint detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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