Hot-Start DNA Polymerase, High Fidelity

Hot-Start High-Fidelity Taq DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase engineered for applications requiring exceptional accuracy and reliability. The enzyme features a ‘hot-start’ mechanism, where antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start enhances sensitivity, specificity, and yield, while enabling reaction assembly at room temperature for added convenience. With fidelity approximately 50 times higher than standard Taq polymerase, Hot-Start High-Fidelity Polymerase ensures precise DNA amplification, making it ideal for high-fidelity PCR and the generation of blunt-end PCR products for cloning.

Hot-Start High-Fidelity Taq DNA Polymerase efficiently amplifies targets up to 5–10 kb in size, even from low- and high-complexity templates, and demonstrates high resistance to inhibitors, ensuring robust performance across a variety of challenging sample types. The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction and features 3'→5' exonuclease (proofreading) activity, which corrects nucleotide incorporation errors during DNA synthesis. This proofreading capability significantly enhances the fidelity and accuracy of DNA polymerization. Additionally, the enzyme lacks 5'→3' exonuclease activity and reverse transcriptase activity, ensuring the production of blunt-end PCR products suitable for downstream applications like blunt cloning.