Robust thermostable polymerase for routine PCR
Standard 5′→3′ DNA polymerase with low 5′→3′ exonuclease activity and no proofreading. Adds 3′‑A overhangs for TA cloning. Reliable amplification across typical templates and buffers for everyday PCR and genotyping.
| Product Number | EM15TAQDNA |
| Function | 5' -> 3' polymerase; Low 5' -> 3' exonuclease activity; No proofreading |
| For Use In | Routine PCR; Genotyping; Colony PCR; TA cloning |
| Molecular Weight | 94 kDa |
| Concentration | 5 U/µL |
| Delivery Format | Tubes |
| Intended Use | This product is intended for research use only |
| Volume | 100 µL 500 µL |
| Source | Recombinant E. coli |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20°C |
| Stability | ≥12 months |
Taq DNA Polymerase is a thermostable enzyme originally derived from the thermophilic bacterium Thermus aquaticus. It catalyzes the 5' to 3' synthesis of DNA and is widely recognized for its robust performance in a variety of PCR applications. Known for its robust performance and versatility, Taq Polymerase is a trusted choice for routine PCR, RT-PCR, and TA cloning workflows. Its ability to deliver high product yields from a variety of templates, combined with its compatibility with both standard and fast PCR protocols, makes it an essential tool for molecular diagnostics and research laboratories.
We offer flexible customization to meet your specific needs:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 72°C in the presence of the reaction buffer.
Functional assay
Human genomic DNA was amplified using the DNA Polymerase and specific primers to produce a distinct band of 750 bp.
Self-priming activity
Standard PCR is carried out without primers, using the DNA Polymerase and human genomic DNA. No products were amplified.
Exonuclease assay
Linearized lambda/HindII fragments are incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No degradation of DNA was observed.
Endonuclease assay
lambda DNA is incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No degradation of DNA was observed.
Nick Activity
Supercoiled plasmid DNA is incubated with the DNA Polymerase in a 50 µl reaction mixture for 4 h at 37°C. No conversion of covalently closed circular DNA to nicked DNA was detected.
E. coli DNA contamination assay
A sample of the denatured DNA Polymerase is analyzed with specific primers targeting the 16S rRNA gene in qPCR for the presence of contaminating E. coli DNA. No E. coli DNA was detectable.
No. The optimal temperature for Taq polymerase to work is 72°C. If you need to run rapid, ambient-temperature DNA or RNA amplification, explore our RPA offerings.
The optimal primer concentration depends on the application, but 0.2–1 µM of each primer (forward and reverse) is commonly used in PCR reactions and is also the range recommended in the user guide. We highly recommend optimizing primer concentration based on your experimental design. Using too little primer can reduce amplification efficiency and yield, while excessive primer concentrations may increase nonspecific amplification and primer-dimer formation.
The enzyme and its buffer system are optimized for a slightly higher melting temperature, providing researchers with a higher starting point to aim for better specificity and sensitivity for assays in general.
Since each assay is unique, we strongly recommend performing a gradient PCR as part of the optimization to identify the most suitable annealing temperature for your specific design.
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