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- Enhances RNA Yield: Hydrolyzes pyrophosphate to shift equilibrium toward RNA synthesis.
- Safe and Reliable: Animal-free production for therapeutic applications.
- Optimal Conditions: Requires Mg²⁺ as a cofactor and performs best at pH 7.0.
Boost mRNA yield by removing pyrophosphate
In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions.
| Product Number | R25PYROPHOS |
| Function | Hydrolyzes pyrophosphate to phosphate; relieves product inhibition |
| For Use In | in vitro transcription (IVT) yield enhancement, RNA synthesis optimization |
| Concentration | 0.1 U/µL |
| Molecular Weight | 33.4 kD |
| Source | Recombinant E. coli |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20u00b0C |
| Purity | ≥ 95% |
| Delivery Format | Tubes |
| Intended Use | This product is intended for research use only |
Pyrophosphatase, Inorganic is a high-quality enzyme designed to hydrolyze inorganic pyrophosphate generated during in vitro transcription (IVT) reactions. By removing unwanted pyrophosphate, the enzyme shifts the reaction equilibrium toward RNA synthesis, significantly increasing RNA yield. This makes it an essential tool for mRNA production in both research and therapeutic applications.
We offer flexible customization to meet your specific needs:
One unit is defined as the amount of enzyme that will generate 1 μmol of orthophosphate per minute from inorganic pyrophosphate (PPi) in a 10 minute standard reaction at 25°C containing 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, and 2 mM PPi.
Purity (SEC-HPLC): ≥95.0%
Endotoxin: ≤10.0 EU/ml
Residual RNase: Negative
Activity (Enzyme Catalytic Efficiency): ≥0.1 U/μl