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PRODUCT

SpCas9 Nuclease-mRNA

Gene Editing With High Precision

SpCas9 mRNA encodes the widely used CRISPR nuclease for efficient genome editing in mammalian cells. Delivery of mRNA enables rapid, transient Cas9 expression that reduces persistent nuclease exposure while supporting robust editing when paired with synthetic sgRNAs in research workflows.

  • Enables efficient gene knockout and precise sequence modification
  • Pairs with synthetic gRNAs for high on-target editing performance
  • Supports transient nuclease expression for controlled editing activity
  • Suitable for ex vivo cell engineering and in vivo genome editing research

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SpCas9 Nuclease-mRNA (1 mg)

#M20SPCAS9-Lg
Description Deliverables Specifications gRNA Design Resources
Description

SpCas9 mRNA for Controlled Gene Editing

SpCas9 is a well-characterized CRISPR nuclease that enables precise genome editing through RNA-guided DNA cleavage at NGG PAM sites. It generates site-specific blunt double-strand breaks, supporting gene knockout and targeted genome modification via endogenous DNA repair pathways, including non-homologous end joining (NHEJ) and homology-directed repair (HDR).

Delivered as mRNA, SpCas9 enables transient intracellular expression following translation, eliminating the need for recombinant protein delivery. The expressed nuclease associates with synthetic single guide RNA (sgRNA) to form an active ribonucleoprotein complex, enabling efficient and predictable genome editing across diverse cell types.

Image
RNP complex schematic of SpCas9. Depicted in the schematic is genomic DNA (light blue), PAM sequence (pink), and predicted cut site locations on genomic DNA (gray scissors). Nuclease is depicted in light green/blue gradient color. The guide RNA or gRNA is comprised of the target sequence (green) and scaffold (dark blue).

Key Features

Controlled Expression:
Enables transient intracellular expression of SpCas9, supporting time-limited nuclease activity.

Wide Targeting Capability:
Recognizes NGG PAM sequences, enabling targeting across a large portion of the genome.

Established System:
Encodes a widely used and well-characterized nuclease with consistent performance across editing applications.

Deliverables

Product Number M20SPCAS9
Concentration 1 µg/µL
Delivery Format Tubes
Intended Use This product is intended for research use only
Source in vitro transcription
Shipping Conditions Dry Ice
Storage Temperature -80 C
Buffer Composition 1 mM Sodium Citrate, pH 6.4
Function Catalyzes site-specific double-stranded DNA cleavage guided by a single guide RNA (sgRNA) to facilitate targeted genome engineering in eukaryotic cells
For Use In CRISPR-Cas9 gene editing experiments with nuclease mRNA
*Synthego sources materials from multiple qualified suppliers with buffer concentrations within this range.

Specifications

Specification SpCas9
Size 4522 nt
PAM Sequence (N = any nucleotide) 5'-NGG-3’
DNA Cleavage Blunt end cut 3nt upstream of PAM sequence
Endonuclease Domains HNH and RuvC
gRNA Length 97 - 103nt
Target Sequence Length 20 nt
Enzyme Class Type IIa CRISPR-Cas system of Streptococcus pyogenes Cas9
Guide RNA

Designing SpCas9 guide RNA

For designing SpCas9 guide RNA(s), use your favorite guide design platform and select SpCas9 nuclease, which has a 5'-NGG PAM sequence.

After you design your SpCas9 guide RNA, use our easy-to-use order interface to order your guides. We will synthesize them resulting in high-quality synthetic guide RNA you complex with our SpCas9 nuclease to achieve your highest editing efficiency.

The following modifications are included on our SpCas9 sgRNA during synthesis:

2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases

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