SpCas9 nuclease mRNA and gRNA User Guide
A resource to get started using your SpCas9 nuclease mRNA and gRNA
mRNA format
This User Guide covers important guidelines for storage, preparation, and getting started with our SpCas9 nuclease mRNA and gRNA.
SpCas9 is the industry-standard CRISPR nuclease, widely used for reliable and efficient genome editing across research applications. Delivered in mRNA format, SpCas9 enables transient nuclease expression, while maintaining strong on-target activity. Supported by extensive validation across cell types, SpCas9 nuclease mRNA and gRNA provide a flexible, scalable solution for advancing your gene editing workflows.
This User Guide provides step-by-step support for working with SpCas9 nuclease mRNA and guide RNA (gRNA), including:
- Storage recommendations for SpCas9 nuclease mRNA and gRNA
- Instructions for gRNA preparation and handling
- Recommended gRNA controls for SpCas9 nuclease mRNA
- Key tips for efficient delivery and transfection
- Guidance on evaluating and interpreting editing outcomes
Download the SpCas9 nuclease mRNA and gRNA User Guide to accelerate your next therapeutic innovation.