PRODUCT

Recombinase Polymerase Amplification (RPA) Endpoint Kit

Rapid isothermal DNA amplification at 37–42 °C

Lyophilized 2× master mix for endpoint detection delivers highly sensitive amplification in ~10–20 minutes without thermal cycling. Streamlined single-step reconstitution for easy integration and rapid assay development.

Fast results in ~10–20 minutes
37–42 °C low‑temperature amplification
Lyophilized 2× mix for room temp stability
Endpoint detection via gel or lateral flow

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Recombinase Polymerase Amplification (RPA) Endpoint Kit (8 rxn)

#MM32RPABASE-Sm

Specifications Description Quality FAQ Resources

Product Number	MM32RPABASE
Function	RPA chemistry for endpoint detection
For Use In	Gel or Lateral-Flow Detection
Source	Recombinant E. coli
Shipping Conditions	Ambient
Storage Temperature	Ambient
Stability	≥12 months
Delivery Format	Tubes
Intended Use	This product is for research use only
Components	LyoBead RPA Endpoint; 2X RPA Reconstitution Buffer; 20X RPA Reaction Initiator

Rapid, Isothermal DNA Amplification for Endpoint Analysis

The RPA Endpoint Kit is a ready-to-use, high-performance solution for ultra-rapid DNA amplification. Designed to operate at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific DNA detection in as little as 10 to 20 minutes. Its lyophilized master mix format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

The kit is optimized for robust performance across a variety of sample types, including crude extracts, and is compatible with multiple readout methods such as lateral flow and electrophoresis. RPA kits require a single reconstitution step, streamlining integration into developing workflows and making it an excellent choice for time-critical diagnostics, pathogen detection, and environmental testing.

Key Features

Ready-to-Use Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration. Perfect for point-of-care and remote settings.
Ultra-Rapid Amplification: Achieves highly sensitive DNA detection in just 10–20 minutes.
Robust Performance: Delivers high efficiency and specificity across diverse sample types, including crude extracts.
Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

Pathogen Detection: Rapid and reliable amplification of low-copy-number DNA for clinical diagnostics.
Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
Assay Development: Scalable nucleic acid testing from research to high-throughput workflows.
Flexible Readouts: Compatible with lateral flow, electrophoresis, and other endpoint detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

Frequently Asked Questions

What is Recombinase Polymerase Amplification (RPA) and how does it work?

Recombinase polymerase amplification, or RPA, is a remarkable isothermal DNA amplification method that does not need thermal cyclers, operates at 37-42 ºC, and provides exponential amplification of the targets.

The RPA process begins when recombinase proteins bind to primers and search for matching sequences in double-stranded DNA, enabling strand invasion and stabilization by single-stranded binding proteins. After the recombinase disassembles, a strand-displacing DNA polymerase elongates the primer, and repeated cycles of this reaction lead to exponential DNA amplification. For more information, refer to our comprehensive guide to the mechanisms and methods of RPA.

What types of samples and targets can I use with RPA Endpoint kits?

Our RPA Endpoint Kits are greatly suited for highly sensitive DNA detection in just 10-20 minutes for point-of-care and low-resources, field-based settings where performance, speed, and flexibility are key. A wide range of sample types are compatible with the RPA Endpoint kits, including crude extracts in addition to purified nucleic acids.

What materials and equipment do I need for RPA?

To run an RPA endpoint reaction with our kits, you’ll need:

A pair of forward and reverse primers
Nuclease-free water
Pipettes with sterile tips
Sterile tubes
Gloves

Depending on your specific method of endpoint detection, you may also need detection probes.

A heat block, water bath, or incubator is sufficient for a controlled, constant reaction temperature between 37 ºC and 42 ºC. A regular PCR thermal cycler works, too.

For endpoint detection, you’ll need a compatible signal detection system. Common options include:

Colorimetric detection, commonly adopted in lateral flow assays
Electrophoresis-based detection
Other endpoint detection methods suited to your experimental design

Can I use PCR primers for RPA assays?

For optimal results, it is recommended to extend the length of target-specific PCR primers for the use in RPA experiments.

Optimal RPA primers for our kits should be between 30 and 35 nt with a GC content between 30 and 60%. They should not be longer than 45 nt. Hence, RPA primers are slightly longer than common PCR primers (usually 18 to 30 nt). Considerations for reducing secondary structures (e.g., hairpins) and primer-dimer formations in primer design apply to RPA primers, too.

Please also design the primers so that the amplicons are between 80 and 200 bp, and should not exceed 400 bp, to achieve efficient and robust amplification.

What primer concentration should I use for RPA?

Optimal final primer concentration should be determined through titration testing for each specific assay. A good starting point is usually 0.4 – 0.5 µM of each primer in a 50-µL reaction, and one should adjust based on the assay performance.

RESOURCES