Delivering CRISPR components in the ribonucleoprotein (RNP) format, pre-complexed Cas9 and synthetic sgRNA, in cells yields consistent high indel frequencies with low off-target effects. Download this collaboration poster to see the editing results from multiple cell types.

Studies show that a ribonucleoprotein (RNP) complex between Cas9 nuclease and synthetic sgRNA produces the most consistent genome editing, highest indel frequencies with the lowest off-target effects, and no risk of DNA incorporation compared to plasmid or mRNA-based approaches.

This poster examines the following:

  • Value of RNP Formation
  • More Consistent Guides with Synthetic RNA
  • Two-Piece vs. One-Piece Systems
  • Functional Validation Across Cell Types
  • Importance and Potential Benefits of Chemical Modification

Download the poster now!