Gene editors, Control sgRNA, Reagents & Add-ons

This positive control is a validated gRNA sequence targeting RELA with editing efficiency of 80% - 90% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.

Includes 1 nmol of modified sgRNA.

This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.

Includes 1 nmol of modified sgRNA.

Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.

This gRNA generates a non-edited control for analyzing SpCas9-edited cells.

Includes 1 nmol of modified sgRNA.

Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.

This gRNA generates a non-edited control for analyzing SpCas9-edited cells.

Includes 1 nmol of modified sgRNA.

This positive control is a validated gRNA sequence targeting Rosa26 in mouse cell lines. Use this control to optimize transfection conditions for maximum editing efficiency with SpCas9.

Includes 1 nmol of modified sgRNA.

This multi-guide positive control is a validated trio of gRNAs targeting TRAC in human cell types.

For the full kit of the positive control with the addition of sequencing and PCR primers as well as Cas9 nuclease, purchase the Transfection Optimization Kit.

Includes 1.5 nmol of modified multi-guide sgRNA.

These validated gRNA controls are engineered for the eSpOT-ON nuclease system, enabling precise assessment of cleavage efficiency and specificity. Designed to match eSpOT-ON kinetics, they provide a reliable benchmark for optimizing gene editing workflows.

Includes eSpOT-ON nuclease mRNA, eSpOT-ON nuclease protein, 1.5 nmol B2M gRNA, 1.5 nmol TRAC gRNA, and 1.5 nmol NTC gRNA.

This validated controls kit is engineered for SpCas9 gene editing in human cells. It enables the precise assessment of cleavage efficiency and specificity by providing benchmark positive and negative gRNAs targeting a known locus. These controls are essential for optimizing transfection or electroporation protocols, validating sgRNA delivery, and quantifying on-target indel formation for reliable data normalization.

• Validate SpCas9 editing activity with positive and negative controls for human gene targets.
• Establish a benchmark for quantifying on-target cleavage efficiency and indel formation.
• Normalize experimental data to distinguish delivery failure from ineffective gRNA design.

Formulated with 10mM Tris-HCl and 1mM EDTA at pH 8.0, this TE Buffer protects nucleic acids from degradation during long-term storage. Ideal for solubilizing nucleic acids, diluting samples, and washing precipitates in molecular biology protocols.

Includes 5x 1mL tubes

Prepared for critical molecular biology workflows, this nuclease-free water ensures RNA stability. Validated for use in CRISPR gRNA preparation, RNA extraction, cDNA synthesis, and ligation.

Includes 5x 1mL tubes