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PRODUCT

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit

Real-time fluorescent RT-RPA for RNA targets (exo)

Integrates reverse transcription with real-time RPA for rapid RNA target quantification. 2× lyophilized master mix supports easy workflow integration with standard instruments. Real‑time detection via exo probes at 37–42 °C with minimal hands‑on time.

  • Exo‑probe real‑time fluorescence
  • Minimal hands‑on time for reaction at 37–42 °C
  • Lyophilized 2× mix for easy workflow integration
  • Ideal for rapid RNA target quantification

Select a Size

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit (8 rxn)

#MM32RTRPAEXO-Sm

Product Specifications

Product Number MM32RTRPAEXO
Function RT-RPA with exo probes for real-time fluorescence
For Use In Rapid RNA target quantification
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Sensitive and Specific RNA Detection in Real Time

The RT-RPA Real-Time Kit is a ready-to-use, high-performance solution for real-time RNA amplification under isothermal conditions. Operating at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific RNA detection in as little as 10 to 20 minutes. Its lyophilized format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Powered by a proprietary reverse transcriptase optimized for isothermal amplification, the kit ensures accurate and efficient cDNA synthesis. It is formulated for fluorescence-based real-time detection, delivering strong signal clarity and enabling real-time monitoring of amplification with minimal hands-on time. Its robust performance across diverse sample types, including crude extracts, makes it a versatile tool for time-critical diagnostics and research applications.

Key Features

  • Ready-to-Use Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration. Perfect for point-of-care and remote settings.
  • Ultra-Rapid Amplification: Achieves sensitive RNA detection in just 10–20 minutes.
  • Proprietary Reverse Transcriptase: Engineered for efficient cDNA synthesis at isothermal temperatures.
  • Real-Time Monitoring: Optimized for fluorescence-based detection with strong signal clarity.
  • Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.
  • Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
  • Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

  • Pathogen Detection: Rapid and reliable low-copy RNA amplification for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
  • Assay Development: Scalable RNA amplification from research to high-throughput workflows.
  • Flexible Readouts: Compatible with real-time fluorescence, lateral flow, and other detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional Assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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