PRODUCT

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit

Real-time fluorescent RT-RPA for RNA targets (exo)

Integrates reverse transcription with real-time RPA for rapid RNA target quantification. 2× lyophilized master mix supports easy workflow integration with standard instruments. Real‑time detection via exo probes at 37–42 °C with minimal hands‑on time.

Exo‑probe real‑time fluorescence
Minimal hands‑on time for reaction at 37–42 °C
Lyophilized 2× mix for easy workflow integration
Ideal for rapid RNA target quantification

Select a Size

Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Real-Time RNA Detection Kit (8 rxn)

#MM32RTRPAEXO-Sm

Specifications Description Quality FAQ Resources

Product Number	MM32RTRPAEXO
Function	RT-RPA with exo probes for real-time fluorescence
For Use In	Rapid RNA target quantification
Source	Recombinant E. coli
Shipping Conditions	Ambient
Storage Temperature	Ambient
Stability	≥12 months
Delivery Format	Tubes
Intended Use	This product is for research use only

Description

Sensitive and Specific RNA Detection in Real Time

The RT-RPA Real-Time Kit is a ready-to-use, high-performance solution for real-time RNA amplification under isothermal conditions. Operating at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific RNA detection in as little as 10 to 20 minutes. Its lyophilized format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Powered by a proprietary reverse transcriptase optimized for isothermal amplification, the kit ensures accurate and efficient cDNA synthesis. It is formulated for fluorescence-based real-time detection, delivering strong signal clarity and enabling real-time monitoring of amplification with minimal hands-on time. Its robust performance across diverse sample types, including crude extracts, makes it a versatile tool for time-critical diagnostics and research applications.

Key Features

Ready-to-Use Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration. Perfect for point-of-care and remote settings.
Ultra-Rapid Amplification: Achieves sensitive RNA detection in just 10–20 minutes.
Proprietary Reverse Transcriptase: Engineered for efficient cDNA synthesis at isothermal temperatures.
Real-Time Monitoring: Optimized for fluorescence-based detection with strong signal clarity.
Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.
Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

Pathogen Detection: Rapid and reliable low-copy RNA amplification for clinical diagnostics.
Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
Assay Development: Scalable RNA amplification from research to high-throughput workflows.
Flexible Readouts: Compatible with real-time fluorescence, lateral flow, and other detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

Volume: Optimize packaging sizes to match your application scale.
Concentration: Customize concentrations for optimal performance.
Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional Assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

Frequently Asked Questions

What is Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) and how does it work?

Reverse transcription recombinase polymerase amplification, or RT-RPA, is a remarkable isothermal RNA amplification method that does not need thermal cyclers, operates at 37-42ºC, and provides exponential amplification of the targets.

The RT-RPA process integrates reverse transcription to convert the target region to cDNA first, then RPA process begins when recombinase proteins bind to primers and search for matching sequences along the cDNA to hybridize. After the recombinase disassembles, a strand-displacing DNA polymerase elongates the primer, and repeated cycles of this reaction lead to exponential RNA target amplification. For more information, refer to our comprehensive guide to the mechanisms and methods of RT-RPA.

What types of samples and targets can I use with RT-RPA Real-Time kits?

Our RT-RPA Real-Time RNA Detection Kits are greatly suited for highly sensitive, real-time quantification of RNA targets in under 30 minutes. They are optimized for point-of-care and field-based applications, providing strong signal clarity with minimal hands-on time. The kits demonstrate exceptional versatility, showing compatibility with a wide range of sample types including crude extracts.

What materials and equipment do I need for RT-RPA?

To perform an RT-RPA real-time reaction with our kits, you’ll need:

A pair of forward and reverse primers
An Exo probe for real-time signal detection with your choice of fluorophores (e.g., FAM, HEX, etc)
Nuclease-free water
Pipettes with sterile tips
Sterile tubes
Gloves

Equipment:
A heat block, water bath, or incubator is sufficient for a controlled, constant reaction temperature between 37ºC and 42ºC. A regular PCR thermal cycler works, too.

For real-time detection, you’ll need a fluorescence signal detection instrument. Our RT-RPA Real-Time RNA Detection Kits are compatible with a wide range of such instruments, including regular real-time PCR systems. If you choose to use endpoint detection methods for the RT-RPA Real-Time reaction products, common options include:

Colorimetric detection, commonly adopted in lateral flow assays
Electrophoresis-based detection
Other endpoint detection methods suited to your experimental design

Can I use PCR primers for real-time RT-RPA assays?

For optimal results, it is recommended to extend the length of target-specific PCR primers for the use in real-time RT-RPA experiments.

Optimal real-time RT-RPA primers for our kits should be between 30 and 35 nt with a GC content between 30 and 60%. They should not be longer than 45 nt. Hence, RT-RPA primers are slightly longer than common PCR primers (usually 18 to 30 nt). Considerations for reducing secondary structures (e.g., hairpins) and primer-dimer formations in primer design apply to RT-RPA primers, too.

Please also design the real-time RT-RPA primers so that the amplicons are between 110 and 200 bp, and should not exceed 400 bp, to achieve efficient and robust amplification.

Please design the Exo probe so that it has a length between 48 and 52 nt. Due to the use of such a probe for real-time measurement, our RT-RPA Real-Time RNA Detection Kits have a slightly longer amplicon recommendation than the RT-RPA Endpoint RNA Detection Kits.

What primer concentration should I use for real-time RT-RPA assays?

Optimal final primer and probe concentrations should be determined through titration testing for each specific assay. A good starting point is usually 0.4 – 0.5 µM of each primer and ~ 0.12 µM of the probe in a 50-µL reaction, and one should adjust the concentrations based on the assay performance.

RESOURCES