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PRODUCT

Recombinase Polymerase Amplification (RPA) Real-Time Kit

Real-time fluorescence-enabled RPA (exo probes)

2× lyophilized master mix supports real‑time detection via exo probes at 37–42 °C with minimal hands‑on time. Ideal for rapid DNA target quantification and workflow integration with standard instruments.

  • Exo‑probe real‑time fluorescence
  • Minimal hands‑on time at 37–42 °C
  • Lyophilized 2× mix; easy integration
  • Ideal for rapid DNA target quantification

Select a Size

Recombinase Polymerase Amplification (RPA) Real-Time Kit (8 rxn)

#MM32RPAEXO-Sm

Product Specifications

Product Number MM32RPAEXO
Function RPA with exonuclease and fluorescent probes
For Use In Real-Time DNA target quantification
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Real-Time DNA Amplification with Fluorescence Monitoring

The RPA Real-Time Kit is a ready-to-use, high-performance solution for ultra-rapid, real-time DNA amplification under isothermal conditions. Operating at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific DNA detection in as little as 10-20 minutes. Its lyophilized master mix format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

Optimized for fluorescence-based detection, the kit allows real-time monitoring of amplification with strong signal clarity and minimal hands-on time. Its robust formulation ensures efficient target recognition and consistent performance, even with complex or unprocessed sample types, making it a reliable choice for time-critical diagnostics and research applications.

Key Features

  • Ready-to-Use Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration. Perfect for point-of-care and remote settings.
  • Ultra-Rapid Amplification: Achieves highly sensitive DNA detection in just 10–20 minutes.
  • Real-Time Monitoring: Optimized for fluorescence-based detection with strong signal clarity.
  • Robust Performance: High efficiency and specificity across diverse sample types, including crude extracts.
  • Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
  • Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

  • Pathogen Detection: Rapid and reliable amplification of low-copy-number DNA for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
  • Assay Development: Scalable nucleic acid detection from research to high-throughput workflows.
  • Flexible Readouts: Compatible with real-time fluorescence, lateral flow, and other detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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