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PRODUCT

Recombinase Polymerase Amplification (RPA) Endpoint Kit

Rapid isothermal DNA amplification at 37–42 °C

Lyophilized 2× master mix for endpoint detection delivers fast amplification in ~10–20 minutes at low constant temperatures. Streamlined setup suits gel or lateral‑flow readouts and rapid assay development.

  • Fast results in ~10–20 minutes
  • 37–42 °C low‑temperature amplification
  • Lyophilized 2× mix simplifies setup
  • Endpoint detection via gel or lateral flow

Select a Size

Recombinase Polymerase Amplification (RPA) Endpoint Kit (8 rxn)

#MM32RPABASE-Sm

Product Specifications

Product Number MM32RPABASE
Function RPA chemistry for endpoint detection
For Use In Gel or Lateral-Flow Detection
Source Recombinant E. coli
Shipping Conditions Ambient
Storage Temperature Ambient
Stability ≥12 months
Delivery Format Tubes
Intended Use This product is for research use only
Description

Fast Isothermal DNA Amplification for Endpoint Analysis

The RPA Endpoint Kit is a ready-to-use, high-performance solution for ultra-rapid DNA amplification. Designed to operate at constant low temperatures (37°C–42°C), this kit eliminates the need for thermal cycling, enabling sensitive and specific DNA detection in as little as 10 to 20 minutes. Its lyophilized master mix format ensures stability for ambient transport and storage, making it ideal for point-of-care and field-based applications.

The kit is optimized for robust performance across a variety of sample types, including crude extracts, and is compatible with multiple readout methods such as lateral flow and electrophoresis. RPA kits require a single reconstitution step, streamlining integration into developing workflows and making it an excellent choice for time-critical diagnostics, pathogen detection, and environmental testing.

Key Features

  • Lyophilized Master Mixes: Stable for ambient storage and transport, eliminating the need for refrigeration. Perfect for point-of-care and remote settings.
  • Ultra-Rapid Amplification: Achieves highly sensitive DNA detection in just 10–20 minutes.
  • Robust Performance: Delivers high efficiency and specificity across diverse sample types, including crude extracts.
  • Isothermal Operation: Functions at constant low temperatures (37°C–42°C), removing the need for thermal cycling.
  • Customizable Components: Tailor high-purity reagents to your unique assay conditions.

Applications

  • Pathogen Detection: Rapid and reliable amplification of low-copy-number DNA for clinical diagnostics.
  • Environmental Testing: Ideal for monitoring contaminants using portable devices in field-based settings.
  • Assay Development: Scalable nucleic acid testing from research to high-throughput workflows.
  • Flexible Readouts: Compatible with lateral flow, electrophoresis, and other endpoint detection methods.

Custom Component Options

We offer flexible customization to meet your specific needs:

  • Volume: Optimize packaging sizes to match your application scale.
  • Concentration: Customize concentrations for optimal performance.
  • Formulation: Available in liquid and lyophilized formats to suit your protocols.

Quality

Individual kit components are tested before and after formulation and post-lyophilization.

Functional assay
The performance of the reconstituted LyoBead is confirmed by an RPA reaction.

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