RNase-free DNase I clears DNA from RNA workflows
RNase‑free DNase I rapidly digests plasmid templates after IVT and removes genomic DNA from RNA preps. Protect RNA integrity and prevent false positives in RT‑qPCR and RNA‑seq with a high‑activity enzyme compatible with common buffers.
| Product Number | R20DNASEI |
| Function | Endonuclease cleaving ssDNA and dsDNA into oligonucleotides |
| For Use In | DNA removal from IVT mRNA prep; gDNA clearance from RNA isolations |
| Concentration | 4 U/µL |
| Delivery Format | Tubes |
| Intended Use | This product is intended for research use only |
| Heat Inactivation | 10 min @ 65°C |
| Purity | ≥ 95% |
| Volume | 0.25 mL 1.2 mL |
| Source | Recombinant E. coli |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20°C |
| Stability | 24 Months |
DNase I (also referred to as DNase 1) is a versatile endonuclease that efficiently digests both single- and double-stranded DNA into oligonucleotides with 5´-phosphate and 3´-hydroxylated ends. This enzyme is essential for applications requiring precise DNA removal, ensuring high-quality RNA samples and reliable downstream results.
DNase I is widely used in RNA purification workflows, where it eliminates contaminating DNA to improve the accuracy of RNA-based applications. It is also ideal for in vitro transcription (IVT) processes and DNase I footprinting, making it a valuable tool for molecular diagnostics and mRNA synthesis.
We offer flexible customization to meet your specific needs:
One unit is the amount of enzyme required to completely degrade 1 μg of pBR322 DNA in 10 minutes at 37°C in DNase I reaction buffer.
Purity (SEC-HPLC): ≥95.0%
Activity (Plasmid Degradation): ≥4 U/μl
Endotoxin: ≤10.0 EU/ml
Residual RNase: Negative
After IVT, DNase I removes the DNA template used for transcription. This ensures DNA-free RNA products for downstream applications. Learn more in our IVT guide: The Ultimate IVT Optimization and Troubleshooting Checklist.
Most applications of DNase involve cleaning up RNA samples from DNA contaminants, such as from genomic DNA contaminants in RNA samples. Using an RNase-free DNase I helps ensure DNA contaminants are removed without compromising RNA integrity.
One unit is equal to the enzyme required to completely degrade 1 μg of pBR322 DNA in 10 minutes at 37 °C in DNase I reaction buffer.
No. DNase I specifically targets DNA and does not degrade RNA.
Cart
Account
Your Orders
Sign Out
Sign In