RT optimized for low‑copy and structured RNAs
High‑sensitivity reverse transcriptase designed for highly specific, low‑copy detection and structured RNAs. Excels in standard RT, RT-PCR, RT‑qPCR, RNA analysis by primer extension, and RACE with clean backgrounds and consistent yields.
| Product Number | EM16HSREVT |
| Function | Engineered RT optimized for specificity/sensitivity |
| For Use In | RT-qPCR; Low-copy RNA detection; RACE; Primer extension |
| Concentration | 4 U/µL |
| Delivery Format | Tubes |
| Intended Use | This product is intended for research use only |
| Volume | 100 µL 500 µL |
| Source | Recombinant E. coli |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20°C |
| Stability | ≥12 months |
High Sensitivity Reverse Transcriptase is a proprietary enzyme designed for highly specific and sensitive reverse transcription, delivering exceptional performance across a wide range of RNA analysis applications. With its high affinity for RNA, it efficiently transcribes complex RNA secondary structures and targets in low copy numbers, resulting in high yields of cDNA. This multifunctional enzyme combines RNA-dependent and ssDNA-dependent DNA polymerase activities with RNase H activity that is specific to RNA hybridized to cDNA. This specificity ensures no degradation of pure RNA templates, improving the performance of subsequent PCR steps. High Sensitivity Reverse Transcriptase is ideal for standard reverse transcription polymerase chain reaction (RT-PCR), qRT-PCR, and advanced workflows like RACE and primer extension RNA analysis.
To ensure RNA integrity and prevent contamination during cDNA synthesis:
We offer flexible customization to meet your specific needs:
One unit is the amount of enzyme activity that incorporates 1 nmol of dTTP into acid insoluble fraction in 10 minutes at 42°C when poly(A)+ RNA and oligo(dT)20 are used as template–primer.
Exonuclease assay
Linearized lambda/HindIII fragments are incubated with the Reverse Transcriptase in a 50 µl reaction mixture for 16 h at 37°C. No degradation of DNA was observed.
Endonuclease/Nick Activity
Supercoiled plasmid DNA is incubated with the enzyme in a 50 µl reaction mixture for 4 h at 37°C. No conversion of covalently closed circular DNA to nicked DNA detected.
Contamination with E. coli DNA
Absence of E. coli genomic DNA is confirmed by qPCR using a sample of the enzyme and specific primers targeting the E. coli 16S rRNA gene. No contamination detected.
RNase Assay
An RNA template is incubated with the enzyme in a 20 µl reaction mixture for 1 h at 42°C. No RNA degradation observed.
Functional Assay
cDNA synthesis with Oligo (dT) and/or Hexamer primers, followed by PCR.
High Sensitivity Reverse Transcriptase is useful when working with low-abundance RNA, limited RNA input, or challenging RNA templates where maximizing cDNA yield is important.
High-quality template RNA is critical for efficient cDNA synthesis, especially when working with low-abundance transcripts or limited sample inputs. Optimizing primer design and reaction conditions, in addition to preserving RNA integrity, can help maximize cDNA yield and sensitivity. High Sensitivity Reverse Transcriptase is designed to support efficient cDNA synthesis from challenging samples where increased sensitivity is required.
For RNA templates that form challenging secondary structures, you can pre-incubate your RNA samples for 5 minutes at 65°C (place on ice immediately after). Consult our user guide for more information.
Explore our reverse transcription guide to learn about reverse transcription polymerase chain reaction (RT – PCR)
Cart
Account
Your Orders
Sign Out
Sign In