This guide covers storage, usage, and troubleshooting for our RNase Inhibitor — everything you need for reliable RNase protection across RNA workflows.

Storage: Store at –20°C in the supplied buffer (20 mM HEPES, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6). Supplied at 40 U/µL. Minimize freeze-thaw cycles.

Usage: Add 1–2 U per 1 µL of reaction mixture, before or alongside any RNase-prone components. Optimal activity at 37°C; a minimum of 1 mM DTT is required to keep the enzyme active. To inactivate, heat to 65°C for 20 minutes.

Troubleshooting: The full guide includes fixes for six common issues: RNA degradation, low RNA yield in IVT or cDNA synthesis, inconsistent cDNA synthesis, ineffective inhibitor activity, residual RNase activity in purified RNA, and inhibitor overuse.

Download the full guide for complete usage recommendations and the troubleshooting table.