Plasmid and virus-mediated delivery is a common way to introduce CRISPR-Cas9 editing machinery into cells. Sorting the edited cells from unmanipulated ones often requires selectable markers. Unfortunately, the use of selection agents can pose various drawbacks to your cells, thus compromising your experimental outcomes.

As a result, it’s important that you set up your CRISPR editing project properly by utilizing the right materials, procedures, and strategies that do not negatively impact your cell line. Luckily, Synthego has developed high-quality synthetic guides and a robust RNP delivery method for generating gene edits while maintaining the integrity of your cell-based model.

Learn how Synthego can help you set up your CRISPR editing project for success. We cover the following:

  • The dangers of utilizing chemical selection
  • Synthego’s process for delivering CRISPR-Cas9 machinery
  • The benefits of non-selection-based CRISPR formats

Download this flyer on the Dangers of Selection Agents today!