This guide covers storage conditions, reaction setup, and troubleshooting for Synthego's T7 RNA Polymerase — everything you need for consistent, high-yield in vitro transcription. For enzyme specifications and quality control data, see the product page.

Storage: Store at –20°C in the supplied buffer (50 mM Tris-HCl, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9). Supplied at 50 U/µL.

Reaction setup: A standard 20 µL transcription reaction runs at 37°C for 1–2 hours (extend to 4–8 hours for transcripts ≤100 nt). The full guide walks through reagent order, mixing steps, and optional DNase I treatment to remove template DNA post-transcription.

Troubleshooting: The guide includes fixes for the five most common issues: low yield, RNA degradation, high dsRNA content, short/fragmented RNA, and incomplete transcription — each with likely causes and recommended solutions.

Download the full guide for complete reagent lists, step-by-step protocol, and the troubleshooting table.