How to Order Research sgRNA in Plate Format

Ordering multiple guide RNA (gRNA) sequences? Synthego's plate ordering option makes it easy to configure and order synthetic gRNAs in a convenient 96-well plate format.

Whether you're screening multiple targets, testing different guide sequences, or scaling your CRISPR experiments, this step-by-step guide walks you through preparing your plate design, uploading sequences, reviewing your configuration, and completing your order.

Our bulk upload feature allows you to add 5–96 sequences at once, helping simplify the ordering process and reduce manual data entry.

Note: Plates have to be delivered with a consistent gene editor selection as well as a consistent nmol size.

Ready to get started? Start your plate order. 

Before You Order

To ensure a smooth ordering experience, complete these steps before starting your order:

• Create or sign in to your Synthego account.
  You’ll need to be signed in to place an order. Need an account? Start here.
• Decide on your CRISPR nuclease or base editor.
  Your choice determines which guide RNA format you’ll need.
  For more information, see our guide to choosing the right nuclease for your application.
• Identify your target sequence.
  Use a CRISPR guide design tool to generate your target sequence.
  You can find some of our favorite free design tools here.
• Determine your plate requirements.
  Confirm the number of sequences, desired well positions, and required gRNA quantities before beginning.

Step 1: Open the Ordering Portal and Select Plates

You can open our ordering portal by clicking the “Order” menu and selecting “Research sgRNA” and clicking “order now” from the Product Page.

Overview of Synthego’s gRNA ordering portal

Here is a quick overview of the various sections of our online gRNA ordering portal.

Once inside the configurator, select "Plates" to begin setting up your order.

Important: You must select the Plates option before uploading your file. If this option is not selected, your order will default to tube format.

Step 2: Download the Plate Ordering Template

The easiest way to configure a plate order is through Synthego's bulk upload feature, which allows you to upload between 5 and 96 sequences using a CSV file.

There are two ways to prepare your file:

Option 1: Download the Sample CSV Template

Download our Sample Plate Ordering Template – .XLSX to access a preformatted file with example values and required fields.

Note: This example file is designed to show available options to use within the excel file. You will need to modify the values to match your needs, knowing that a plate must use one consistent "Gene Editor" selection and one consistent "Size" selection.

Option 2: Create Your Own Template

If you prefer, manually enter a few sequences into the configurator, then select "Download" in the upper-right corner to export your current configuration as a CSV file.

You can then use this file as a starting point for the rest of your plate design.

Step 3: Complete Your Plate Configuration File

Once you've downloaded your CSV template, populate the required fields for each guide RNA sequence.

Each row represents an individual well in your plate. Make sure all entries follow the accepted formats and configuration requirements outlined below.

Additional Configuration Guidance

Plate Positions

The template supports a standard 96-well plate layout, with positions ranging from A01 to H12. If you're ordering fewer than 96 sequences, simply remove the unused rows from your CSV file.

Guide RNA Sequences

Sequences should preferably be entered in RNA notation (A, U, C, G). If your sequences are entered using DNA notation (A, T, C, G), the configurator provides an option to convert them to RNA after upload.

When using a supported Synthego gene editor, enter only the target-specific guide sequence. Do not include the PAM or scaffold sequence.

If you select "Other" as your gene editor, you must provide the complete guide RNA sequence, including the scaffold and any required chemical modifications.

For additional information on sequence length, orientation, and modification requirements, visit our Research sgRNA Ordering Guide.

Chemical Modifications

For eSpOT-ON, hfCas12Max, and AccuBase, select "Standard" to use the Synthego-optimized modifications designed for the selected editor.

For SpCas9, Standard and None (unmodified) options are available.

When using "Other" as your gene editor, select "Other" for chemical modifications and specify any required modifications directly within the sequence field.

Step 4: Upload Your Plate Configuration

Once your CSV file is complete, return to the Research sgRNA configurator.

1. Confirm that "Plates" is selected.
2. Navigate to the bottom of the configuration page.
3. Click "Bulk Upload Sequence."
4. Select your completed CSV file to begin the upload.

The configurator will process your file and populate the plate configuration using the information provided.

Step 5: Review and Troubleshoot Your Plate Order

After uploading your CSV file, review your plate configuration to confirm that all sequences and specifications have been entered correctly.

Before proceeding, verify the following:

• Plate layout: Confirm that sequences are assigned to the intended well positions.
• Sequence information: Check sequence names, lengths, and nucleotide notation.
• Gene editor compatibility: Verify that the selected gene editor and chemical modifications are appropriate for each guide.
• Quantity and purification: Confirm the correct nmol amount and purification method.

Troubleshooting Upload Errors

If your file contains errors or unsupported configurations, the configurator will display errors and warnings at the bottom of the page.

Common items to review include:

• Missing or incorrectly formatted required fields
• Invalid sequence lengths or nucleotide notation
• Unsupported gene editor and chemical modification combinations
• Incorrect plate positions or configuration values

You can manually adjust sequence information and configuration details directly within the configurator to resolve any issues.

Once all errors have been addressed and your plate configuration is correct, you're ready to proceed.

Step 6: Add Your Plate to Cart and Complete Checkout

After reviewing your plate configuration, select "Add to Cart" to proceed with your order.

From your shopping cart, you can review your order details and complete checkout using the available purchasing options, including requesting a quote or purchasing directly with a credit card.

Once your order is accepted, Synthego will provide an estimated shipment timeline based on your plate configuration and order specifications.

Ready to Order Your Plate?

Simplify your CRISPR workflow with convenient plate ordering and bulk sequence upload. Whether you're evaluating multiple guide sequences or preparing for larger screening experiments, Synthego makes it easy to configure your synthetic gRNAs in one order.

Start Your Plate Order

Need additional assistance? Explore our Help Center, review our Research sgRNA Ordering Guide, or contact the Synthego team for support.