High-specificity ligase for RNA end-joining
T4 RNA Ligase II (also called T4 RNA Ligase 2) joins 3'-OH RNA to 5'-phosphorylated oligos within duplex contexts, reducing adapter dimers and artifacts. It supports dsRNA nick sealing and RNA circularization at various scales, and produces cleaner small RNA libraries.
| Product Number | R34T4RNALIG |
| Function | Joins 3'-OH RNA to 5'-phosphorylated oligos in duplex context |
| For Use In | Small RNA-Seq 3' adapter ligation; RNA circularization; dsRNA nick repair |
| Concentration | 50 U/µL |
| Delivery Format | Tubes |
| Intended Use | This product is intended for research use only |
| Heat Inactivation | Not recommended, may denature dsRNA, terminate w/Prot. K or EDTA |
| Purity | ≥ 95% |
| Volume | 20 µL 100 µL |
| Source | Recombinant E. coli |
| Shipping Conditions | Cold Pack |
| Storage Temperature | -20°C |
| Stability | 24 Months |
T4 RNA Ligase II is an ATP-dependent RNA ligase that catalyzes the formation of phosphodiester bonds, enabling both inter- and intramolecular RNA strand joining. This enzyme is specifically optimized for joining nicks on double-stranded RNA (dsRNA) and is significantly more active in this function compared to T4 RNA Ligase I. Additionally, it can ligate the 3´-OH of RNA to the 5´-phosphate of DNA in a double-stranded structure, making it a versatile tool for RNA and DNA manipulation.
We offer flexible customization to meet your specific needs:
One unit is defined as the amount of enzyme required to ligate 0.4 μg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction of 20 μl in 30 minutes at 37°C.
Purity (Bis-Tris PAGE): ≥95.0%
Purity (SEC-HPLC): ≥95.0%
Activity: ≥50 U/μl
Residual RNase: Negative in T4 RNA Ligase II; Negative in 10X T4 RNl2 Reaction Buffer)
Choose T4 RNA Ligase II when the ends you want to join are held together in a duplex or other structured context. Examples include a nick in dsRNA or an RNA 3′ OH adjacent to a 5′-phosphorylated DNA oligo in a double-stranded structure. For sealing nicks in dsRNA, T4 RNA Ligase II is significantly more active than T4 RNA Ligase I. It also has much higher activity on dsRNA nicks than on joining free single-stranded RNA ends, typically making T4 RNA Ligase I the better choice for typical ssRNA-to-ssRNA ligation.
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